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Plant Molecular Biology

, Volume 66, Issue 5, pp 475–490 | Cite as

Co-regulation of nuclear genes encoding plastid ribosomal proteins by light and plastid signals during seedling development in tobacco and Arabidopsis

  • Daniel MacLean
  • Cheryl A. Jerome
  • Anthony P. C. Brown
  • John C. Gray
Article

Abstract

Genes encoding plastid ribosomal proteins are distributed between the nuclear and plastid genomes in higher plants, and coordination of their expression is likely to be required for functional plastid protein synthesis. A custom microarray has been used to examine the patterns of accumulation of transcripts from plastid and nuclear genes encoding plastid ribosomal proteins during seedling development in tobacco and Arabidopsis. The transcripts accumulate coordinately during early seedling development and show similar responses to light and to inhibitors, such as norflurazon and lincomycin, affecting plastid signaling. Computational analysis of the promoters of these genes revealed a shared initiator motif and common cis-elements characteristic of photosynthesis genes, specifically the GT-1 element, and the I-box. Analysis of the RPL27 gene of Arabidopsis thaliana indicated that transcription initiates from an initiator-like region. Deletion analysis of the RPL27 promoter in transgenic plants revealed that the identified shared cis-elements were not all required for wild-type expression patterns, and full developmental, light- and plastid-regulation can be conveyed by a region of the promoter from −235 to +1 relative to the transcription start site.

Keywords

Initiator element Lincomycin Microarray Norflurazon Plastid ribosomal protein RPL27 

Abbreviations

GUS

β-Glucuronidase

MU

4-Methylumbelliferone

MUG

4-Methylumbelliferyl β-d-glucuronide

RACE

Rapid amplification of cDNA ends

X-gluc

5-Bromo-4-chloro-3-indolyl β-d-glucuronide

Notes

Acknowledgements

We thank Dr. Christine Newell and Dr. Juliet Coates for guidance on plant transformation and GUS assays, and Sue Aspinall for technical assistance. DM was supported by a Research Studentship from the Biotechnology and Biological Sciences Research Council.

Supplementary material

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Daniel MacLean
    • 1
    • 2
  • Cheryl A. Jerome
    • 1
    • 3
  • Anthony P. C. Brown
    • 1
    • 4
  • John C. Gray
    • 1
  1. 1.Department of Plant SciencesUniversity of CambridgeCambridgeUK
  2. 2.Sainsbury LaboratoryJohn Innes CentreColneyUK
  3. 3.Centre for Indigenous Environmental ResourcesWinnipegCanada
  4. 4.Department of PathologyUniversity of CambridgeCambridgeUK

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