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Plant Molecular Biology

, Volume 64, Issue 5, pp 575–587 | Cite as

AtCYT-INV1, a neutral invertase, is involved in osmotic stress-induced inhibition on lateral root growth in Arabidopsis

  • Xiaopeng Qi
  • Zhongchang Wu
  • Jinhui Li
  • Xiaorong Mo
  • Shihua Wu
  • Jun Chu
  • Ping Wu
Article

Abstract

Neutral/Alkaline invertases are unique to plant and photosynthetic bacteria. The function of Neutral/Alkaline invertases in plant development is not clear so far. In this study, we isolated an Arabidopsis (Col-0) mutant insensitive to osmotic stress-induced inhibition on lateral root growth. Map-based cloning reveals that a neutral invertase gene (AtCYT-INV1) was point-mutated. The mutant Atcyt-inv1 showed short primary root, smaller size of leaves and siliques, and promotion of the reproductive compared to the wild type (WT). Carbohydrate measurement showed that sucrose is accumulated and glucose is reduced in the mutant Atcyt-inv1 under normal and 3% mannitol treatments. Taken together, AtCYT-INV1 plays multiple roles in plant development and is involved in osmotic stress-induced inhibition on lateral root growth by controlling the concentration of hexose in cells.

Keywords

ABA AtCYT-INV1 Lateral root Neutral invertase Osmotic stress 

Notes

Acknowledgements

We thank Dr Peter Doerner for generous gifts of the transgenic Arabidopsis seeds expressing CycB1 promoter-GUS. This research was supported by the National Basic Research Program of China (973) (No. 2005CB120901).

Supplementary material

11103_2007_9177_MOESM1_ESM.doc (324 kb)
(DOC 324 kb)

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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Xiaopeng Qi
    • 1
  • Zhongchang Wu
    • 1
  • Jinhui Li
    • 1
  • Xiaorong Mo
    • 1
  • Shihua Wu
    • 1
  • Jun Chu
    • 1
  • Ping Wu
    • 1
  1. 1.The State Key Laboratory of Plant Physiology and Biochemistry, College of Life SciencesZhejiang UniversityHangzhouPR China

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