Plant Molecular Biology

, Volume 58, Issue 6, pp 775–787 | Cite as

Identification of a Nicotiana plumbaginifolia Plasma Membrane H+-ATPase Gene Expressed in the Pollen Tube

  • Benoit Lefebvre
  • Miguel Arango
  • Mohammed Oufattole
  • Jérôme Crouzet
  • Bénédicte Purnelle
  • Marc Boutry


In Nicotiana plumbaginifolia, plasma membrane H+-ATPases (PMAs) are encoded by a gene family of nine members. Here, we report on the characterization of a new isogene, NpPMA5 (belonging to subfamily IV), and the determination of its expression pattern using the β-glucuronidase (gusA) reporter gene. pNpPMA5gusA was expressed in cotyledons, in vascular tissues of the stem (mainly in nodal zones), and in the flower and fruit. In the flower, high expression was found in the pollen tube after in vitro or in vivo germination. Northern blotting analysis confirmed that NpPMA5 was expressed in the pollen tube contrary to NpPMA2 (subfamily I) or NpPMA4 (subfamily II), two genes highly expressed in other tissues. The subcellular localization of PM H+-ATPase in the pollen tube was analyzed by immunocytodecoration. As expected, this enzyme was localized to the plasma membrane. However, neither the tip nor the base of the pollen tube was labeled, showing an asymmetrical distribution of this enzyme. This observation supports the hypothesis that the PM H+-ATPase is involved in creating the pH gradient that is observed along the pollen tube and is implicated in cell elongation. Compared to other plant PM H+-ATPases, the C-terminal region of NpPMA5 is shorter by 26 amino acid residues and is modified in the last 6 residues, due to a sequence rearrangement, which was also found in the orthologous gene of Nicotiana glutinosa, a Nicotiana species distant from N. plumbaginifolia and Petunia hybrida and Lycopersicon esculentum, other Solanacae species. This modification alters part of the PM H+-ATPase regulatory domain and raises the question whether this isoform is still regulated.


asymmetric distribution H+-ATPase plasma membrane pollen pollen tube transport 


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Copyright information

© Springer 2005

Authors and Affiliations

  • Benoit Lefebvre
    • 1
  • Miguel Arango
    • 1
  • Mohammed Oufattole
    • 1
  • Jérôme Crouzet
    • 1
  • Bénédicte Purnelle
    • 1
  • Marc Boutry
    • 1
  1. 1.Unité de biochimie physiologique, Institut des sciences de la vieUniversité catholique de LouvainLouvain-la-NeuveBelgium

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