Comprehensive approach to genes involved in cell wall modifications in Arabidopsis thaliana
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The plant cell wall is of supermolecular architecture, and is composed of various types of heterogeneous polymers. A few thousand enzymes and structural proteins are directly involved in the construction processes, and in the functional aspects of the dynamic architecture in Arabidopsis thaliana. Most of these proteins are encoded by multigene families, and most members within each family share significant similarities in structural features, but often exhibit differing expression profiles and physiological functions. Thus, for the molecular dissection of cell wall dynamics, it is necessary to distinguish individual members within a family of proteins. As a first step towards characterizing the processes involved in cell wall dynamics, we have manufactured a gene-specific 70-mer oligo microarray that consists of 765 genes classified into 30 putative families of proteins that are implicated in the cell wall dynamics of Arabidopsis. By using this array system, we identified several sets of genes that exhibit organ preferential expression profiles. We also identified gene sets that are expressed differentially at certain specific growth stages of the Arabidopsis inflorescence stem. Our results indicate that there is a division of roles among family members within each of the putative cell wall-related gene families.
Key wordsArabidopsis cell wall DNA microarray enzyme gene expression stem
reverse transcription-polymerase chain reaction
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- Ariizumi, T., Amagai, M., Shibata, D., Hatakeyama, K., Watanabe, W. and Toriyama, K. 2002. Comparative study of promoter activity of three anther-specific genes encoding lipid transfer protein, xyloglucan endotransglucosylase/hydrolase and polygalacturonase in transgenic Arabidopsis thaliana. Plant Cell Rep. 21: 90–96.CrossRefGoogle Scholar
- Bourquin, V., Nishikubo, N., Abe, H., Brumer, H., Denmen, S., Eklund, M., Christiermin, M., Teeri, T.T., Sundberg, B. and Mellerowicz, E.J. 2002. Xyloglucan endotransglycosylases have a function during the formation of secondary cell walls of vascular tissues. Plant Cell 14: 3073–3088.CrossRefPubMedGoogle Scholar
- Brownleader, M.D., Hopkins, J., Mobasheri, A., Dey, P.M., Jackson, P. and Trevan, M. 2000. Role of extensin peroxidase in tomato (Lycopersicon esculentum Mill) cells in suspension culture. Planta 191: 457–469.Google Scholar
- Coutinho, P.M. and Henrissat, B. 1999. Carbohydrate-active enzymes: an integrated database approach. In: H.J. Glbert, G. Davies, B. Henrissat and B. Svensson (Eds.), “Recent Advances in Carbohydrate Bioengineering”, The Royal Society of Chemistry. Cambridge, pp. 3–12.Google Scholar
- Dong, X., Mindrinos, M., Daviqb, K.R. and Asubel, F.M. 1991. Induction of Arabidopsis defense genes by virulent and avirulent Psudomonas syringae strains and by a cloned avirulence gene. Plant Cell 65: 57–60.Google Scholar
- Hyodo, H., Yamakawa, S., Takeda, Y., Tsuduki, M., Yokota, A., Nishitani, K. and Kohchi, T. 2003. Active gene expression of a xyloglucan endotransglucosylase/hydrolase gene, XTH9, in inflorescence apices is related to cell elongation in Arabidopsis thaliana. Plant Mol. Biol. 52: 473–482.CrossRefPubMedGoogle Scholar
- Johnson K.L., Jones B.J., Schultz C.J. and Bacic A. 2003. Nonenzyme cell wall (glyco)proteins. In: J.K.C. Rose (Ed.) The Plant Cell Wall (ISBN 0-8493-2811-X) CRC Press LLC, FL, USA, pp. 111–154.Google Scholar
- Mayfield, J.A., Fiebig, A., Johnstone, S.E. and Preuss, D. 2001. Gene families from the Arabidopsis thaliana pollen coat proteome. Nature 292: 2482–2485.Google Scholar
- Sander, L., Child, R., Ulvskov, P., Albrechtsen, M. and Borkhardt, B. 2001. Analysis of dehiscence zone endopolygalacturonase in oil seed rape (Brassica napus) and Arabidopsis thaliana: evidence for roles in cell separation in dehiscence and abscission zones, and in stylar tissues during pollen tube growth. Plant Mol. Biol. 46: 469–479.CrossRefPubMedGoogle Scholar
- Yokoyama, R. and Nishitani, K. 2001a. A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis. Plant Cell Physiol. 42: 1025–1033.CrossRefPubMedGoogle Scholar