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Plant Molecular Biology

, Volume 60, Issue 3, pp 365–376 | Cite as

Cloning and Characterization of a Second form of the Rice Adenine Phosphoribosyl Transferase Gene (OsAPT2) and its Association with TGMS

  • Chun-Jiang Zhou
  • Jun Li
  • Ji-Cheng Zou
  • Feng-Shan Liang
  • Chun-Jiang Ye
  • De-Min Jin
  • Man-Li Weng
  • Bin Wang
Article

Abstract

A rice gene, OsAPT2, which encodes a putative adenine phosphoribosyl transferase (APRT), was cloned and characterized. Analysis of the cDNA and genomic sequences revealed seven exons and six introns in the OsAPT2. The deduced amino acid sequence of OsAPT2 is highly homologous to those of previously isolated APRTs. RT-PCR analysis indicated that the OsAPT2 transcript in the young panicles of ‘Annong S-1’ is down-regulated at 29 °C, the critical temperature for induction of ‘Annong S-1’ fertility conversion. Since the panicle is likely the thermo-sensitive organ at the early stages of pollen fertility alternation, the observed heat-induced change in the OsAPT2 expression pattern in young panicles may mediate, at least in part, thermo-sensitive genic male sterility (TGMS) in ‘Annong S-1’. An antisense strategy was used to suppress the expression of the OsAPT2 homolog in Arabidopsis, and the obtained homozygous transgenic plants contained lower AMP content, displayed lower pollen germination rates and exhibited some abnormalities in leaf phenotypes and flowering timing. These data suggest that OsAPT2 is likely to be involved in TGMS in the rice line ‘Annong S-1’.

Keywords

adenine phosphoribosyl transferase (APRT) Arabidopsis thaliana Oryza sativa thermo-sensitive genic male sterility (TGMS) 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Chun-Jiang Zhou
    • 1
    • 2
    • 3
  • Jun Li
    • 1
    • 3
  • Ji-Cheng Zou
    • 1
  • Feng-Shan Liang
    • 1
    • 3
  • Chun-Jiang Ye
    • 1
    • 3
  • De-Min Jin
    • 1
  • Man-Li Weng
    • 1
  • Bin Wang
    • 1
  1. 1.State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina
  2. 2.College of Life SciencesHebei Normal UniversityShijiazhuangChina
  3. 3.Graduate School of the Chinese Academy of SciencesBeijingChina

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