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Plant Molecular Biology

, Volume 56, Issue 6, pp 849–861 | Cite as

Molecular characterization of a WEE1 gene homologue in tomato (Lycopersicon esculentum Mill.)

  • Nathalie Gonzalez
  • Michel Hernould
  • Frédéric Delmas
  • Frédéric Gévaudant
  • Philippe Duffe
  • Mathilde Causse
  • Armand Mouras
  • Christian Chevalier
Article

Abstract

Early fruit development in tomato (Lycopersicon esculentum Mill.) proceeds in two distinct phases of growth that comprise cell division and cell expansion, respectively. In pericarp and the jelly like locular tissue of tomato fruit, the transition between cell division to cell expansion is characterized by the arrest of mitotic activity, numerous rounds of nuclear DNA endoreduplication and the inhibition of Cyclin-Dependent Kinase A (CDKA) activity. To investigate whether the WEE1 kinase may play a role during the endoreduplication process, we isolated and characterized the tomato homologue for WEE1. The LeWEE1 gene consisted of 10 exons with a predicted 510 amino acid-long protein. The accumulation of the corresponding transcripts was associated with mitotically active organs: developing fruits, seeds and roots. Interestingly, LeWEE1was expressed in the jelly like locular tissue concomitant with endoreduplication during fruit development. Using tobacco BY-2 synchronized cells, we showed that the WEE1 gene expression is cell-cycle regulated with a maximum transcript accumulation at S phase. Our data indicate the putative dual contribution of LeWEE1 in the classical cell cycle and the endocycle.

Keywords

cell division cell expansion early fruit development endoreduplication tomato WEE1 

Abbreviations:

CDK

cyclin dependent kinase

DPA

days post anthesis

MG

mature green

RR

red ripe

RT

reverse transcription

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Copyright information

© Springer 2005

Authors and Affiliations

  • Nathalie Gonzalez
    • 1
  • Michel Hernould
    • 1
  • Frédéric Delmas
    • 1
  • Frédéric Gévaudant
    • 1
  • Philippe Duffe
    • 2
  • Mathilde Causse
    • 2
  • Armand Mouras
    • 1
  • Christian Chevalier
    • 1
  1. 1.Unité Mixte de Recherche en Physiologie et Biotechnologie Végétales Institut de Biologie Végétale Intégrative, Institut National de la Recherche AgronomiqueInstitut National de la Recherche Agronomique et Universités de Bordeaux 1 et Victor Segalen-Bordeaux 2CedexFrance
  2. 2.Unité de Génétique et Amélioration des Fruits et LégumesInstitut National de la Recherche AgronomiquecedexFrance

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