Plant Molecular Biology

, Volume 55, Issue 6, pp 797–805 | Cite as

Activation of the oxidative burst by yeast elicitor in Catharanthus roseus cells occurs independently of the activation of genes involved in alkaloid biosynthesis

  • Bea Pauw
  • Bert van Duijn
  • Jan W. Kijne
  • Johan Memelink


In Catharanthus roseus cell suspensions, expression of several terpenoid indole alkaloid (TIA) biosynthetic genes, including those encoding strictosidine synthase and tryptophan decarboxylase, is coordinately induced by fungal elicitors such as yeast extract (YE). This induction is mediated by several signaling steps including the biosynthesis of jasmonic acid, and the activation of the jasmonic acid-responsive ORCA transcription factors. We investigated a possible role of reactive oxygen species (ROS) as a second messenger in this system. YE was shown to activate the production of ROS, which was dependent on protein phosphorylation and calcium influx. However, ROS generation was neither necessary for the induction of genes involved in TIA biosynthesis by YE nor by itself sufficient to induce these genes. Therefore, we conclude that activation of the oxidative burst by YE occurs independently of the activation of genes involved in TIA biosynthesis.

Catharanthus roseus elicitor jasmonic acid reactive oxygen species (ROS) secondary metabolism terpenoid indole alkaloid 


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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Bea Pauw
    • 1
  • Bert van Duijn
    • 2
  • Jan W. Kijne
    • 1
  • Johan Memelink
    • 1
  1. 1.Institute of BiologyLeiden UniversityLeidenNetherlands
  2. 2.Department of Applied Plant SciencesThe Netherlands Organisation for Applied Scientific ResearchLeidenNetherlands

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