Photosynthetica

, Volume 56, Issue 1, pp 316–321 | Cite as

Effect of green light on the amount and activity of NDH-1–PSI supercomplex in Synechocystis sp. strain PCC 6803

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Abstract

Cyanobacterial NDH-1 interacts with PSI to form NDH-1–PSI supercomplex. CpcG2, a linker protein for the PSI-specific peripheral antenna CpcG2-phycobilisome, is essential for stabilization of the supercomplex. Green light (GL) increased the expression of CpcG2 but had little effect, if any, on the expression of NDH-1 and PSI, when compared to the abundance of these components under red light (RL). The increased expression of CpcG2 intensified the band of NDH-1–PSI supercomplex after blue-native gel electrophoresis of the thylakoid membrane, possibly by stabilizing the supercomplex. The activity of NDH-1-dependent cyclic electron transport around PSI increased when cells grown under RL were transferred to a low intensity GL but was suppressed when cells were grown under high intensities of GL. The functionality of PSI showed the same trend. We thus conclude that GL increases the expression of CpcG2, thereby increasing the abundance of the NDH-1–PSI supercomplex and its activity at low GL but not at higher GL.

Additional key words

chlorophyll fluorescence cyanobacteria cyclic electron transport around PSI light-harvesting antenna light quality P700 analysis 

Abbreviations

BN-PAGE

blue-native-PAGE

CBB

Coomassie Brilliant Blue

Chl

chlorophyll

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethylurea

GL

green light

NDH-CET

NDH-1-dependent cyclic electron transport around PSI

PBS

phycobilisome

Pm

the maximal P700 change

RL

red light

Synechocystis 6803

Synechocystis sp. strain PCC 6803

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.College of Life and Environment SciencesShanghai Normal UniversityShanghaiChina
  2. 2.Bioscience CenterNagoya UniversityChikusa, NagoyaJapan

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