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Photosynthetica

, Volume 56, Issue 1, pp 433–444 | Cite as

Improved photosynthesis in Arabidopsis roots by activation of GATA transcription factors

  • A. Ohnishi
  • H. Wada
  • K. Kobayashi
Article

Abstract

Plant cells plastically change their functions according to the environment. Although Arabidopsis roots are heterotrophic organs, they increase photosynthetic capacity after shoot removal. Transcription factors regulating chloroplast development are involved in this response downstream of positive cytokinin and negative auxin regulation. To dissect the crosstalk of these regulators after shoot removal, we analyzed photosynthetic parameters in roots with chloroplast development enhanced by shoot removal, overexpression of transcription factors, or hormonal treatment. Our data suggest that shoot removal improves electron transfer downstream of PSII in roots, with a decrease in nonregulated energy dissipation. Cytokinin, auxin, and transcription factors affect the photosynthetic capacity of roots in a highly complex manner. Overexpression of two different types of transcription factors (GOLDEN 2-LIKE 1 and class-B GATAs) synergistically increased root chlorophyll content while maintaining high photosynthetic efficiency. Our data demonstrate the flexible regulation of the photosynthetic machinery by hormone signaling and downstream transcription factors.

Additional key words

chlorophyll fluorescence effective quantum yield of photosystem II root greening 

Abbreviations

ARR

ARABIDOPSIS RESPONSE REGULATOR

BA

6-benzyladenine

B-GATA

class B GATA transcription factor

Chl

chlorophyll

Fv/Fm

maximal quantum yield of PSII

Fv’/Fm

quantum yield of open PSII under actinic light

GLK

GOLDEN 2-LIKE

GNC

GATA, NITRATE-INDUCIBLE, CARBON METABOLISM INVOLVED

GNL/CGA1

GNC-LIKE/ CYTOKININ-RESPONSIVE GATA TRANSCRIPTION FACTOR 1

IAA

indole 3-acetic acid

MS

Murashige and Skoog

NPQ

nonphotochemical quenching

PAM

pulse amplitude modulation

PCIB

p-chlorophenoxyisobutyric acid

qP

coefficient of photochemical quenching

ΦPSII

effective quantum yield of PSII

ΦNO

quantum yield of nonregulated energy dissipation

ΦNPQ

quantum yield of regulated energy dissipation

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Supplementary material

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.Department of Life Sciences, Graduate School of Arts and SciencesThe University of TokyoMeguro-ku, TokyoJapan

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