Application of spectrally resolved fluorescence induction to study light-induced nonphotochemical quenching in algae
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The light-induced nonphotochemical quenching (NPQ) can safely dissipate excess of absorbed light to heat. Here we describe an application of spectrally resolved fluorescence induction (SRFI) method for studying spectral variability of NPQ. The approach allows detection of spectrally-resolved nonphotochemical quenching (NPQλ) representing NPQ dependency on fluorescence emission wavelength in the whole spectral range of fluorescence emission. The experimental approach is briefly described and NPQλ is studied for the cryptophyte alga Rhodomonas salina and for green alga Chlorella sp. We confirm presence of NPQλ only in membrane-bound antennae (chlorophyll a/c antennae) and not in phycobiliproteins in lumen in cryptophyte and show that NPQλ is inhibited in the whole spectral range by NPQ inhibitors in Chlorella sp. We discuss variability in the quenching in the particular spectral ranges and applicability of the NPQλ parameter to study quenching locus in vivo.
Additional key wordsfluorescence parameters light-harvesting complex photoprotection photosynthesis photosystem II
chlorophyll a/c antennae complexes of cryptophyte
fluorescence intensity at particular irradiance/time of measuring protocol
minimal fluorescence intensity for open reaction center
maximal fluorescence intensity for closed reaction center measured with dark-adapted sample
maximal fluorescence intensity for closed reaction center measured with light-adapted sample
nonphotochemical quenching of chlorophyll a fluorescence
spectrally resolved nonphotochemical quenching of fluorescence
fluorescence decrease ratio
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