Photosynthetica

, Volume 56, Issue 1, pp 265–274 | Cite as

The phycobilisome terminal emitter transfers its energy with a rate of (20 ps)–1 to photosystem II

  • A. M. Acuña
  • P. Van Alphen
  • R. Van Grondelle
  • I. H. M. Van Stokkum
Open Access
Article
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Abstract

Ultrafast time resolved emission spectra were measured in whole cells of a PSI-deficient mutant of Synechocystis sp. PCC 6803 at room temperature and at 77K to study excitation energy transfer and trapping. By means of a target analysis it was estimated that the terminal emitter of the phycobilisome, termed allophycocyanin 680, transfers its energy with a rate of (20 ps)–1 to PSII. This is faster than the intraphycobilisome energy transfer rates between a rod and a core cylinder, or between the core cylinders.

Additional key words

excitation energy transfer global analysis light harvesting target analysis 

Abbreviation

APC

allophycocyanin

DAS

decay-associated spectrum

ΔPSI

PSI-deficient mutant of Synechocystis sp. PCC 6803

EAS

evolution-associated spectrum

ET

electron transfer

EET

excitation energy transfer

FWHM

full width at half maximum

IRF

instrument response function

PB

phycobilisome

PC

phycocyanin

rms

root mean square

RP

radical pair

SAS

species-associated spectrum

SNR

signal to noise ratio

SS

steady-state spectra

SVD

singular value decomposition

TRES

time resolved emission spectrum

WT

wild type

Supplementary material

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Copyright information

© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

Authors and Affiliations

  • A. M. Acuña
    • 1
  • P. Van Alphen
    • 2
  • R. Van Grondelle
    • 1
  • I. H. M. Van Stokkum
    • 1
  1. 1.Institute for Lasers, Life and Biophotonics, Faculty of SciencesVrije Universiteit AmsterdamAmsterdamThe Netherlands
  2. 2.Swammerdam Institute for Life SciencesUniversity of AmsterdamAmsterdamThe Netherlands

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