Abstract
Halomicronema hongdechloris is a chlorophyll (Chl) f-producing cyanobacterium. Chl f biosynthesis is induced under far-red light, extending its photosynthetically active radiation range to 760 nm. In this study, PSI complexes were isolated and purified from H. hongdechloris, grown under white light (WL) and far-red light (FR), by a combination of density gradient ultracentrifugation and chromatographic separation. WL-PSI showed similar pigment composition as that of Synechocystis 6803, using Chl a in the reaction center. Both Chl a and f were detected in the FR-PSI, although Chl f was a minor component (~8% of total Chl). The FR-PSI showed a maximal fluorescence emission peak of 750 nm at 77 K, which is red-shifted ~20 nm compared to the 730 nm recorded from the WL-PSI. The absorption peaks of P700 for WLPSI and FR-PSI were 699 nm and 702 nm, respectively. The function of Chl f in FR-PSI is discussed.
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Abbreviations
- APC:
-
allophycocyanin
- CCA:
-
complementary chromatic adaptation
- Chl:
-
chlorophyll
- FR:
-
730 nm light-emitting diodes
- DoDM:
-
β-dodecyl maltoside
- FaRLiP:
-
far-red light photoacclimation
- FR:
-
far-red light
- FR-PSI:
-
isolated PSI from 730 nm LED-illuminated culture
- OG:
-
n-octyl-β-D-glucopyranoside
- PC:
-
phycocyanin
- TMPD:
-
N,N,N',N'-tetramethyl-p-phenylenediamine
- WL:
-
white fluorescent light
- WL-PSI:
-
isolated PSI from white light culture
- 6803-PSI:
-
purified PSI complexes from Synechocystis 6803
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Acknowledgements: The research was supported by the Australian Research Council Centre of Excellence for translational photosynthesis (CE140100015). The authors thank Dr. Ben Crossette (mass-spectral facility, University of Sydney) and Dr. Miguel A. Hernández-Prieto for assisting with LC-MS/MS and spectral analysis.
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11099_2018_776_MOESM1_ESM.pdf
Fig. 1S. The HPLC chromatographs for the sucrose density band isolated from white light (WL) and far red light (FR) grown Halomicronema hongdechloris. The solid black (FR-2) and grey line (FR-1) represent the chromatographs for the FR band 2 and 1 (Fig.1), respectively. The dash black (WL-2) and grey line (WL-1) represent the chromatographs for the WL band 2 and 1 (Fig.1), respectively. Carotenoids, Chl f, Chl a, Chl a’, β-carotene and pheophytin a are assigned based on their retention time and online spectra.
11099_2018_776_MOESM2_ESM.pdf
Fig. 2S. Sequence alignment for PsaL’s detected from isolated PSI complexes of H. hongdechloris. PsaL (1JB0_L) from PSI crystal structure of Synechococcus elongates is used as a reference. The residues ligating chlorophyll a’s are highlighted by asterisk “*”.
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Li, Y., Vella, N. & Chen, M. Characterization of isolated photosystem I from Halomicronema hongdechloris, a chlorophyll f-producing cyanobacterium. Photosynthetica 56, 306–315 (2018). https://doi.org/10.1007/s11099-018-0776-x
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DOI: https://doi.org/10.1007/s11099-018-0776-x