Analysis of photosystem II electron transfer with natural PsbA-variants by redox polymer/protein biophotoelectrochemistry
- 20 Downloads
Redox polymer/protein biophotoelectrochemistry was used to analyse forward electron transfer of isolated PSII complexes with natural PsbA-variants. PsbA1- or PsbA3-PSII was embedded in a redox hydrogel that allows diffusion-free electron transfer to the electrode surface and thus measurement of an immediate photocurrent response. The initial photocurrent density of the electrode is up to ~2-fold higher with PsbA1-PSII under all tested light conditions, the most prominent under high-light [2,300 μmol(photon) m–2 s–1] illumination with 5 μA cm–2 for PsbA3-PSII and 9.5 μA cm–2 for PsbA1-PSII. This indicates more efficient electron transfer in low-light-adapted PsbA1-PSII. In contrast, the photocurrent decays faster in PsbA1-PSII under all tested light conditions, which suggests increased stability of high-light-adapted PsbA3-PSII. These results confirm and extend previous observations that PsbA3-PSII has increased P680+•/QA–• charge recombination and thus less efficient photon-to-charge conversion, whereas PsbA1-PSII is optimised for efficient electron transfer with limited stability.
Additional key wordsbiophotovoltaics cyanobacteria D1-protein hydrogel
light emitting diode
reaction centre chlorophylls
photosynthetic electron transfer chain
reactive oxygen species
Unable to display preview. Download preview PDF.
- Kato Y., Shibamoto T., Yamamoto S. et al.: Influence of the PsbA1/PsbA3, Ca2+/Sr2+ and Cl-/Br-exchanges on the redox potential of the primary quinone QA in Photosystem II from Thermosynechococcus elongatus as revealed by spectroelectrochemistry.–BBA-Bioenergetics 1817: 1998–2004, 2012b.CrossRefPubMedGoogle Scholar
- Tichý M., Lupı́nková L., Sicora C. et al.: Synechocystis 6803 mutants expressing distinct forms of the Photosystem II D1 protein from Synechococcus 7942. Relationship between the psbA coding region and sensitivity to visible and UV-B radiation.–BBA-Bioenergetics 1605: 55–66, 2003.CrossRefPubMedGoogle Scholar