Photosynthetica

, Volume 56, Issue 1, pp 244–253 | Cite as

Photooxidation and photoreduction of exogenous cytochrome c by photosystem II preparations after various modifications of the water-oxidizing complex

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Abstract

The redox interaction of exogenous cytochrome c550 (Cyt) with PSII isolated from spinach was studied. Illumination of PSII particles in the presence of Cyt led to: (1) Cyt photooxidation by PSII reaction center (demonstrated at the first time), (2) Cyt photoreduction via O2−• photoproduced on the acceptor side of PSII, and (3) Cyt photoreduction by reduced electron carriers of PSII. A step-by-step removal of components of water-oxidizing complex was accompanied by the appearance of Cyt photooxidation, an increase in the superoxide dismutase (SOD)-dependent Cyt photoreduction (related to O2−• formation), and a decrease in the SOD-independent Cyt photoreduction. Re-addition of PsbO protein diminished the Cyt-induced restoration of electron transfer in PSII. Addition of diuron led to inhibition of these photoprocesses, while exogenous Mn2+ inhibited only the Cyt c photooxidation. The results can be important for correct measurements of O2−• photoproduction in PSII and for elucidation of the role of cytochrome c550 in cyanobacterial PSII.

Additional key words

cytochrome c photooxidation photoreduction photosystem II superoxide anion radical 

Abbreviation

Chl

chlorophyll

Cyt

cytochrome

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethylurea

P680

the primary electron donor of PSII

Pheo

pheophytin (the primary electron acceptor of PSII)

RC

reaction center

SOD

superoxide dismutase

TyrZ

redox active tyrosine residue 161 of D1 protein

WOC

water-oxidizing complex

ΔF

photoinduced changes of chlorophyll fluorescence yield of PSII

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  • A. A. Khorobrykh
    • 1
  • D. V. Yanykin
    • 1
  • V. V. Klimov
    • 1
  1. 1.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia

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