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Underestimated chlorophyll a fluorescence measurements on Buxus microphylla red winter leaves

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Photosynthetica

Abstract

Leaves under stressful conditions usually show downregulated maximum quantum efficiency of photosystem II [inferred from variable to maximum chlorophyll (Chl) a fluorescence (Fv/Fm), usually lower than 0.8], indicating photoinhibition. The usual method to evaluate the degree of photoinhibition in winter red leaves is generally by measuring the Fv/Fm on the red adaxial surface. Two phenotypes of overwintering Buxus microphylla ‘Wintergreen’ red leaves, with different measuring site and leaf thickness, were investigated in order to elucidate how red pigments in the outer leaf layer affected the Chl a fluorescence (Fv/Fm) and photochemical reflectance index. Our results showed that the Fv/Fm measured on leaves with the same red surface, but different leaf thickness, exhibited a slightly lower value in half leaf (separated upper and lower layers of leaves by removing the leaf edge similarly as affected by winter freezing and thawing) than that in the intact leaf (without removing the leaf edge), and the Fv/Fm measured on the red surface was significantly lower than that on the inner or backlighted green surface of the same thickness. Our results suggest that the usual measurement of Fv/Fm on red adaxial surface overestimates the actual degree of photoinhibition compared with that of the whole leaf in the winter.

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Abbreviations

Chl:

chlorophyll

Car:

carotenoids

Fm :

maximum fluorescence

F0 :

minimum fluorescence

Fv/Fm :

maximum quantum efficiency of PSII (Fv = Fm - F0)

R/G:

red (adaxial)/green (abaxial)

G/R:

green (adaxial)/red (abaxial)

PRI:

photochemical reflectance index

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Authors and Affiliations

  1. College of Landscape Architecture, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing, China

    J. G. Peng, X. R. Jiang & Y. Liu

  2. College of Architecture and Urban Planning, Guangzhou University, Guangzhou, China

    J. Xu

  3. Beijing Botanical Garden, Beijing, China

    L. Guo

  4. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China

    R. F. Gao

Authors
  1. J. G. Peng
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  2. X. R. Jiang
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  3. J. Xu
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  4. L. Guo
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  5. R. F. Gao
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  6. Y. Liu
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Corresponding author

Correspondence to Y. Liu.

Additional information

Acknowledgments: This work was supported by the Beijing Municipal Science & Technology Commission (Z08050602970801). Authors thank to three reviewers for their valuable comments, which helped improve this manuscript.

J.G. Peng and X.R Jiang contributed equally to this work.

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Peng, J.G., Jiang, X.R., Xu, J. et al. Underestimated chlorophyll a fluorescence measurements on Buxus microphylla red winter leaves. Photosynthetica 55, 561–567 (2017). https://doi.org/10.1007/s11099-016-0660-5

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  • DOI: https://doi.org/10.1007/s11099-016-0660-5

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