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Photosynthetica

, Volume 52, Issue 4, pp 574–580 | Cite as

Toxic effects of erythromycin on photosystem I and II in Microcystis aeruginosa

  • C. -N. Deng
  • D. -Y. Zhang
  • X. -L. Pan
Original Papers

Abstract

Environmental pollution by antibiotics poses a potential ecological risk to aquatic photosynthetic organisms. In the present study, toxic effects of erythromycin on PSI and PSII were investigated in cyanobacteria culture medium of Microcystis aeruginosa. The activity and electron transport of both photosystems were affected by erythromycin in a concentrationdependent manner. The quantum yield of PSII (YII) was reduced at 0.1 mg L−1 of erythromycin, while the quantum yield of PSI (YI) significantly decreased at concentration of 5–25 mg L−1. The decline of YII was accompanied by an increase of nonregulated energy dissipation (YNO). At 10 mg L−1 of erythromycin, YII decreased by 55%, while YNO increased by 18%. The decrease of YI induced by erythromycin was caused by donor-side limitation of PSI (YND). YND was markedly enhanced with elevated erythromycin concentration. At 10 mg L−1 of erythromycin, YI and YNA (PSI acceptor-side limitation) decreased by 8 and 82%, respectively, while YND rose by 314%. The quantum yield of cyclic electron flow increased significantly at 0.1–1 mg L−1 of erythromycin; it decreased but remained higher than that of the control at 5–25 mg L−1 of erythromycin. The contribution of cyclic electron flow to YI, and to linear electron flow rose significantly with the increasing erythromycin concentration. The maximum values of electron transport rates in PSII and PSI decreased by 71 and 24.3%, respectively, at 25 mg L−1 of erythromycin. Compared with the untreated control, the light saturation of PSII and PSI decreased significantly with increasing erythromycin concentration. We showed that concentrations of erythromycin ≥ 5 mg L−1 could exert acute toxicity to cyanobacteria, whereas the chronic toxicity caused by concentrations of ng or μg L−1 needs further research.

Additional key words

chlorophyll fluorescence nonphotochemical quenching photoinhibition 

Abbreviations

CEF

cyclic electron flow

ETR

electron transport rate

ETRI

electron transport rate in PSI

ETRII

electron transport rate in PSII

ETRmax(I)

the maximum electron transport rate in PSI

ETRmax(II)

the maximum electron transport rate in PSII

Ik(I)

the light saturation of PSI

Ik(II)

the light saturation of PSII

LEF

linear electron flow

NPQ

nonphotochemical quenching

RLC

rapid light curves

YCEF

the quantum yield of cyclic electron flow

YCEF/YI

the contribution of cyclic electron flow to YI

YCEF/YII

the ratio of the quantum yield of CEF to LEF

YI

effective photochemical quantum yield of PSI

YII

the effective photochemical quantum yield of PSII

YII/YI

the distribution of quantum yield between two photosystems

YNA

nonphotochemical energy dissipation due to acceptor-side limitation

YND

nonphotochemical energy dissipation due to donor-side limitation

YNO

nonregulated energy dissipation

YNPQ

regulated energy dissipation

αI

the initial slope of RLC of ETRI

αII

the initial slope of RLC of ETRII

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Copyright information

© The Institute of Experimental Botany 2014

Authors and Affiliations

  1. 1.Key Lab of Plateau Lake Ecology & Global Change, College of Tourism and Geographic ScienceYunnan Normal UniversityKunmingChina
  2. 2.State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqiChina
  3. 3.State Key Laboratory of Environmental Geochemistry, Institute of GeochemistryChinese Academy of SciencesGuiyangChina

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