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Photosynthetica

, Volume 52, Issue 4, pp 501–510 | Cite as

Effect of selenium on CO2 and NO3 assimilation under low and adequate nitrogen supply in wheat ( Triticum aestivum L.)

  • R. Hajiboland
  • N. Sadeghzade
Original Papers

Abstract

In order to study the mechanisms of Se-mediated growth improvement as related to carbon (C) and nitrogen (N) metabolism, wheat plants were cultivated hydroponically with adequate (4 mM, Na) or low (1 mM, Nd) N supply and treated with 10 and 50 μM Na2SeO4 for six weeks. The Se supplementation enhanced plant biomass; it was significant for shoots of Na plants at 50 μM Se. Chlorophyll fluorescence parameters were significantly lowered under Nd conditions but restored completely by Se addition reaching values of those in Na plants. Net CO2 assimilation rate (P N) decreased only slightly by limited N availability, but it enhanced significantly in both Nd and Na plants equally by 10 and 50 μM Se. Effect of Se on P N in the Na plants occurred mainly due to the stomata opening, while it was related to both stomatal and nonstomatal mechanisms in the Nd plants. The Se treatment resulted in enhancement of nitrate reductase (NR) activity in both Na and Nd plants with an optimal response at 10 μM Se. Negative correlations between nitrate concentration and NR activity indicated a partial nitrate depletion in the roots following by elevated NR activity in Nd plants. In contrast, nitrite concentrations were higher in the Se treated plants. Higher amino acids and protein concentrations in the Se-treated plants might be an indication of a general upregulation of N metabolism. However, in Na plants, the stimulation of N metabolism was not observed at 50 μM Se which could not be attributed to lesser availability of C skeletons because of maintaning higher CO2 fixation under these conditions. It implies the function of some regulatory mechanisms that are responsible for coordination of C and N metabolism in whole plant.

Additional key words

chlorophyll fluorescence net assimilation rate nitrate reductase nutrients 

Abbreviations

Car

carotenoids

Chl a(b)

chlorophyll a(b)

DM

dry mass

ETR

electron transport rate

Fv′/Fm

excitation capture efficiency of open PSII

F0

initial fluorescence of dark-adapted leaf

FM

fresh mass

E

transpiration rate

Fm

maximum fluorescence of dark-adapted leaf

Fm

maximum fluorescence of light-adapted leaf

Fs

steady-state fluorescence of light-adapted leaf

Fv

variable fluorescence of dark-adapted leaf

Fv/Fm

maximum quantum yield of PSII

gs

stomatal conductance

Na

N-adequate

Nd

N-deficient

NiR

nitrite reductase

NPQ

nonphotochemical quenching

NR

nitrate reductase

PN

net assimilation rate

qP

photochemical quenching

SD

standard deviation

ΦPSII

effective quantum yield of PSII

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Copyright information

© The Institute of Experimental Botany 2014

Authors and Affiliations

  1. 1.Center of Excellence for BiodiversityUniversity of TabrizTabrizIran
  2. 2.Plant Science DepartmentUniversity of TabrizTabrizIran

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