, Volume 50, Issue 3, pp 329–336 | Cite as

Photosynthetic limitations caused by different rates of water-deficit induction in Glycine max and Vigna unguiculata

  • S. C. Bertolli
  • G. L. Rapchan
  • G. M. Souza


Plants are constantly subjected to variations in their surrounding environment, which affect their functioning in different ways. The influence of environmental factors on the physiology of plants depends on several factors including the intensity, duration and frequency of the variation of the external stimulus. Water deficit is one of the main limiting factors for agricultural production worldwide and affects many physiological processes in plants. The aim of this study was to analyse the effects of different rates of induced water deficit on the leaf photosynthetic responses of soybean (Glycine max L.) and cowpea (Vigna unguiculata L.). The plants were subjected to two types of water deficit induction: a rapid induction (RD) by which detached leaves were dehydrated by the exposure to air under controlled conditions and a slow induction (SD) by suspending irrigation under greenhouse conditions. The leaf gas exchange, chlorophyll (Chl) a fluorescence, and relative water content (RWC) were analysed throughout the water-deficit induction. V. unguiculata and G. max demonstrated similar dehydration as the soil water percentage declined under SD, with V. unguiculata showing a greater stomatal sensitivity to reductions in the RWC. V. unguiculata plants were more sensitive to water deficit, as determined by all of the physiological parameters when subjected to RD, and the net photosynthetic rate (P N) was sharply reduced in the early stages of dehydration. After the plants exposed to the SD treatment were rehydrated, V. unguiculata recovered 65% of the P N in relation to the values measured under the control conditions (initial watering state), whereas G. max recovered only 10% of the P N. Thus, the better stomatal control of V. unguiculata could enable the maintenance of the RWC and a more efficient recovery of the P N than G. max.

Additional key words

cowpea photosynthesis rapid and slow water-deficit induction recovery soybean 



transpiration rate


electron transport rate


minimal fluorescence of dark-adapted state


minimal fluorescence of light-adapted state


maximal fluorescence of dark-adapted state


maximal fluorescence of light-adapted state


steady-state fluorescence


variable fluorescence


maximum quantum yield of PSII photochemistry


stomatal conductance


soil water percentage


nonphotochemical quenching


net photosynthetic rate


photochemical quenching coefficient


rapid water-deficit induction


relative water content


slow water-deficit induction


water-use efficiency


effective quantum yield of PSII photochemistry


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  1. Biehler, K., Fock, H.: Evidence for the contribution of mehlerperoxidase reaction in dissipating excess electrons in droughtstressed wheat. — Plant Physiol. 112: 265–272, 1996.PubMedGoogle Scholar
  2. Bilger, W., Björkman, O.: Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis. — Photosynth. Res. 25: 173–185, 1990.CrossRefGoogle Scholar
  3. Bota, J., Medrano, H., Flexas, J.: Is photosynthesis limited by decreased Rubisco activity and RuBP content under progressive water stress? — New Phytol.. 162: 671–681, 2004.CrossRefGoogle Scholar
  4. Câmara, G.M.S., Heiffig, L.S.: [Physiology, environment and yield of soybean.] — In: Câmara, G.M.S (ed.): Soybean: Production Technology. II.: ESALQ/LPV, Piracicaba 2000. [In Portuguese.]Google Scholar
  5. Čatský, J.: Determination of water deficit in discs cut out from leaf blades. — Biol. Plant. 2: 76–77, 1960.CrossRefGoogle Scholar
  6. Catuchi, T. A., Vitolo, H. F., Bertolli, S. C. et al.: Tolerance to water deficiency between two soybean cultivars: transgenic versus conventional. — Ciência Rural 41: 373–378, 2011. [In Portuguese.]CrossRefGoogle Scholar
  7. Chaves, M.M., Oliveira, M.M.: Mechanisms underlying plant resilience to water deficit: prospects for water-saving agriculture. — J. Exp. Bot. 55: 2365–2384, 2004.PubMedCrossRefGoogle Scholar
  8. Cornic, G.: Drought stress inhibits photosynthesis by decreasing stomatal aperture-not by affecting ATP synthesis. — Trends Plant Sci. 5: 187–188, 2000.CrossRefGoogle Scholar
  9. Demmig, B., Björkman, O.: Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution in leaves of higher plants. — Planta 171: 171–184, 1987.CrossRefGoogle Scholar
  10. Doss, B.D., Thulow, D.L.: Irrigation, row width and plant population in relation to growth characteristics of two soybean varieties. — Agron. J. 65: 620–623, 1974.CrossRefGoogle Scholar
  11. Flexas, J., Bota, J., Galmés, J., Medrano, H., Ribas-Carbo, M.: Keeping positive carbon balance under adverse conditions: responses of photosynthesis and respiration to water stress. — Physiol. Plant. 127: 343–352, 2006.CrossRefGoogle Scholar
  12. Genty, B., Briantais, J.M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. — Bioch. Biophys. Acta 990: 87–92, 1989.CrossRefGoogle Scholar
  13. Gopefert, H., Rossetti, L.A., Souza, J.: [Generalized events and agricultural security.] — IPEA, Ministério do Planejamento, Brasília 1993. [In Portuguese.]Google Scholar
  14. Harbison, J., Genty, B., Baker, N.R.: The relationship between CO2 assimilation and electron transport in leaves. — Photosyn. Res. 25: 199–212, 1990.CrossRefGoogle Scholar
  15. Kaiser, W.M.: Effects of water deficit on photosynthetic capacity. — Physiol. Plant. 71: 142–149, 1987.CrossRefGoogle Scholar
  16. Krall, J.P., Edwards, G.E.: Relationship between photosystem II activity and CO2 fixation in leaves. — Physiol. Plant 86: 180–187, 1992.CrossRefGoogle Scholar
  17. Krause, G.H.: Photoinhibition of photosynthesis: an evaluation of damaging and protective mechanisms. — Physiol. Plant. 74: 566–574, 1988.CrossRefGoogle Scholar
  18. Lawlor, D.W., Cornic, G.: Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. — Plant Cell Environ. 25: 275–294, 2002.PubMedCrossRefGoogle Scholar
  19. Lawlor, D.W., Tezara, W.: Causes of decreased photosynthetic rate and metabolic capacity in water-deficient leaf cells: a critical evaluation of mechanisms and integration of processes. — Ann. Bot. 103: 561–579, 2009PubMedCrossRefGoogle Scholar
  20. Leshem, Y.Y., Shewfelt, R.L., Willmer, C.M. et al.: Plant membranes: A biophysical approach to structure, development and senescence. — Kluwer Acad. Publ., Dordrecht 1992.Google Scholar
  21. Matos, M.C., Campos, P.S., Passarinho, J.A. et al.: Drought effect on photosynthetic activity, osmolyte accumulation and membrane integrity of two Cicer arietinum genotypes. — Photosynthetica 48: 303–312, 2010.CrossRefGoogle Scholar
  22. Miyashita, K., Tanakamaru, S., Maitani, T., Kimura, K.: Recovery responses of photosynthesis, transpiration, and stomatal conductance in kidney bean following drought stress. — Environ. Exp. Bot. 53: 205–214, 2005.CrossRefGoogle Scholar
  23. Ögren, E.: Evaluation of chlorophyll fluorescence as a probe for drought stress in willow leaves. — Plant Physiol. 93: 1280–1285, 1990.PubMedCrossRefGoogle Scholar
  24. Oliveira, M.N.S., Oliva, M.A., Martinez, C.A. et al.: [Stomatal sensitivity to ABA related to pH and NO−3, PO4 −3 and Ca+2 levels of xylem sap.] — Braz. J. Plant Physiol. 14: 117–123, 2002. [In Portuguese.]Google Scholar
  25. Parry, M.A. J., Andralojc, P.J., Khan, S. et al.: Rubisco activity: effects of drought stress. — Ann. Bot. 89: 833–839, 2002.PubMedCrossRefGoogle Scholar
  26. Pinheiro, C., Chaves, M.M.: Photosynthesis and drought: can we make metabolic connections from avaiable data? — J. Exp. Bot. 62: 869–882, 2011.PubMedCrossRefGoogle Scholar
  27. Raven, J.A.: The cost of photoinhibition. — Physiol. Plant. 142: 87–104, 2011.PubMedCrossRefGoogle Scholar
  28. Reynolds, M.P., Mujeeb-Kazi, A., Sawkins, M.: Prospects for utilizing plant-adaptive mechanisms to improve wheat and other crops in drought- and salinity-prone environments. — Ann. Appl. Biol. 146: 239–259, 2005.CrossRefGoogle Scholar
  29. Saccardy, K., Pineau, B., Roche, O. et al.: Photochemical efficiency of photosystem II and xanthophyll cycle components in Zea mays leaves exposed to water stress and high light. — Photosynth. Res. 56: 57–66, 1998.CrossRefGoogle Scholar
  30. Scholes, J.D., Press, M.C., Zipperlen, S.W.: Differences in light energy utilisation and dissipation between dipterocarp rain forest tree seedlings. — Oecologia. 109: 41–48, 1997.CrossRefGoogle Scholar
  31. Shangguan, Z.P., Shao, M.G., Dyckmans, J.: Effects of nitrogen nutrition and water deficit on net photosynthetic rate and chlorophyll fluorescence in winter wheat. — J. Plant Physiol. 156: 46–51, 2000.CrossRefGoogle Scholar
  32. Silva, J.M., Arrabaça, M.C.: Photosynthesis in the waterstressed C4 grass Setaria sphacelata is mainly limited by stomata with both rapidly and slowly imposed water deficits. — Physiol. Plant. 121: 409–420, 2004.CrossRefGoogle Scholar
  33. Singh, S. K., Reddy, K. R.: Regulation of photosynthesis, fluorescence, stomatal conductance and water-use efficiency of cowpea (Vigna unguiculata [L.] Walp.) under drought. — J. Photochem. Photobiol. B: Biol. 105: 40–50, 2011.CrossRefGoogle Scholar
  34. Souza, R.P., Machado, E.C., Silva, J.A. et al.: Photosynthetic gas exchange, chlorophyll fluorescence and some associated metabolic changes in cowpea (Vigna unguiculata) during water stress and recovery. — Environ. Exp. Bot. 51: 45–56, 2004.CrossRefGoogle Scholar
  35. Subbarao, G.V., Johansen, C., Slinkard, A.E. et al.: Strategies for improving drought resistance in grain legumes. — Critical Rev. Plant Sci. 14: 469–529, 1995.Google Scholar
  36. Tezara, W., Martínez, D., Rengifo, E. Herrera, A.: Photosynthetic responses of the tropical spiny shrub Lycium nodosum (Solanaceae) to drought, soil salinity and saline spray. — Ann. Bot. 92: 757–765, 2003.PubMedCrossRefGoogle Scholar
  37. Wingler, A., Quick, W.P., Bungard, R.A. et al.: The role of photorespiration during drought stress: an analysis utilizing barley mutants with reduced activities of photorespiratory enzymes. — Plant Cell Environ. 22: 361–373, 1999.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • S. C. Bertolli
    • 1
    • 2
  • G. L. Rapchan
    • 1
  • G. M. Souza
    • 1
  1. 1.Laboratório de Ecofisiologia VegetalUniversidade do Oeste PaulistaPresidente Prudente, SPBrazil
  2. 2.Programa de Pós-graduação em Biologia Vegetal, Instituto de BiociênciasUniversidade Estadual Paulista (UNESP)Rio Claro, SPBrazil

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