, Volume 46, Issue 1, pp 79–83 | Cite as

Temporal characteristics of chlorophyll fluorescence quenching and dissolved oxygen concentration as indicators of photosynthetic activity in Chlorella emersonii

  • N. -E. El Khachia
  • E. O’Mongain
  • A. Collins
Original Papers


A simple chlorophyll fluorescence (CF) measuring system has been implemented to study temporal characteristics of chlorophyll fluorescence induction (CFI) in dark-adapted freshwater algal cultures of Chlorella emersonii. There were two different decay time constants describing the CF quenching: τ0 (the faster) and τ1 (the slower) with amplitudes A0 and A1, respectively. The relative amplitude of the faster quenching component decreased once the sample was subject to deprivation from dissolved oxygen (DO). The DO concentration of samples was monitored to validate the effects of deprivation from air contact for up to 7 d and to the effect of adding DCMU to the culture (herbicide for blocking electron transport of photosystem 2). CFI analysis and DO measurements showed that the relative amplitude of A0 to (A0 + A1) and the DO concentration can be used as an indication of relative photosynthetic activity, thus allowing for the possibility to classify the physiological state of algal blooms into active and inactive states.

Additional key words

algal blooms DCMU non-photochemical quenching photosystem 2 zero oxygen tablets 


A0, A1

the relative fast and slow amplitudes of chlorophyll fluorescence decay


balanced modulator analogue device lock-in amplifier

B0, B1

the inverse of τ0 and τ1, respectively


chlorophyll fluorescence


chlorophyll fluorescence induction




dissolved oxygen




maximal fluorescence yield


fluorescence quenching steady state


light emitting diode


non-photochemical quenching


pulse amplitude fluorometry


photochemical quenching




the total amplitudes of the fast and the slow fluorescence quenching


the amplitude ratio coefficient of the fast to the total quenching (A0/α)

τ0, τ1

the fast and slow chlorophyll fluorescence decay time constants


Zero Oxygen Tablet


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Copyright information

© Institute of Experimental Botany, ASCR 2008

Authors and Affiliations

  • N. -E. El Khachia
    • 1
  • E. O’Mongain
    • 1
  • A. Collins
    • 1
  1. 1.School of PhysicsUniversity College DublinBelfield, Dublin 4Ireland

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