, 45:23 | Cite as

Short-term responses of photosynthetic membrane lipids and photochemical efficiency in plants of Phaseolus vulgaris and Vigna unguiculata submitted to high irradiance

  • L. C. S. Ferreira
  • R. Bressan-Smith
  • T. F. Elias
  • F. F. Silva
  • L. H. Viana
  • J. G. Oliveira
Original Papers


Primary leaves of young plants of common bean (Phaseolus vulgaris cv. Carioca and Negro Huasteco) and cowpea (Vigna unguiculata Walp cv. Epace 10) were exposed to high irradiance (HI) of 2 000 µmol m−2 s−1 for 10, 20, and 30 min. The initial fluorescence (F0) was nearly constant in response to HI in each genotype except for Carioca. A distinct reduction of maximum fluorescence (Fm) was clearly observed in stressed genotypes of beans after 20 min followed by a slight recovery for the longer stress times. In common bean, the maximum quantum yield (Fv/Fm) was reduced slowly from 10 to 30 min of HI. In cowpea, only a slight reduction of Fv/Fm was observed at 20 min followed by recovery to normal values at 30 min. HI resulted in changes in the photochemical (qP) and non-photochemical (qN) quenching in both species, but to a different extent. In cowpea plants, more efficiency in the use of the absorbed energy under photoinhibitory conditions was related to increase in qP and decrease in qN. In addition, lipid peroxidation changed significantly in common bean genotypes with an evident increase after 20 min of HI. Hence the photosynthetic apparatus of cowpea was more tolerant to HI than that of common bean and the integrity of cowpea cell membranes was apparently maintained under HI.

Additional key words

chlorophyll a fluorescence cultivar differences French bean lipid peroxidation photoinhibition photosynthesis species differences 





relative electron transport rate

F0 and Fm

initial and maximum fluorescence


maximum quantum yield


high irradiance


low irradiance




photosynthetic photon flux




quinone A


non-photochemical quenching


photochemical quenching


reaction centre


reactive oxygen species


  1. Aro, E.-M., Virgin, I., Andersson, B.: Photoinhibition of Photosystem II. Inactivation, protein damage and turnover.-Biochim. biophys. Acta 1143: 113–134, 1993.PubMedCrossRefGoogle Scholar
  2. Bolhàr-Nordenkampf, H.R., Long, S.P., Baker, N.R., Öquist, G., Schreiber, U., Lechner, E.G.: Chlorophyll fluorescence as a probe of the photosynthetic competence of leaves in the field: a review of current instrumentation.-Funct. Ecol. 3: 497–514, 1989.CrossRefGoogle Scholar
  3. Buege, J.A., Aust, S.D.: Microsomal lipid peroxidation.-Methods Enzymol. 52: 302–310, 1978.PubMedCrossRefGoogle Scholar
  4. Choudhury, N.K., Behera, R.K.: Photoinhibition of photosynthesis: Role of carotenoids in photoprotection of chloroplast constituents.-Photosynthetica 39: 481–488, 2001.CrossRefGoogle Scholar
  5. Costa, E.S., Bressan-Smith, R., Oliveira, J.G., Campostrini, E., Pimentel, C.: Photochemical efficiency in bean plants during recovery from high temperature stress.-Braz. J. Plant Physiol. 14: 105–110, 2002.CrossRefGoogle Scholar
  6. Dhindsa, R.S., Plumb-Dhindsa, P., Thorpe, T.A.: Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase.-J. exp. Bot. 32: 93–101, 1981.CrossRefGoogle Scholar
  7. Foyer, C.H., Lelandais, M., Kunert, K.J.: Photooxidative stress in plants.-Physiol. Plant. 92: 696–717, 1994.CrossRefGoogle Scholar
  8. Gilmore, A.M., Govindjee: How higher plants respond to excess light: Energy dissipation in photosystem II.-In: Singhal, G.S., Renger, G., Sopory, S.K., Irrgang, K.-D., Govindjee (ed.): Concepts in Photobiology. Pp. 513–548. Kluwer Academic Publ., Boston-Dordrecht-London; Narosa Publishing House, Delhi-Madras-Bombay-Calcutta-London 1999.Google Scholar
  9. Greer, D.H., Berry, J.A., Björkman, O.: Photoinhibition of photosynthesis in intact bean leaves: role of light and temperature, and requirement for chloroplast-protein synthesis during recovery.-Planta 168: 253–260, 1986.Google Scholar
  10. Hideg, É., Murata, N.: The irreversible photoinhibition of the photosystem II complex in leaves of Vicia faba under strong light.-Plant Sci. 130: 151–158, 1997.CrossRefGoogle Scholar
  11. Horton, P., Ruban, A.V., Walter, R.G.: Regulation of light harvesting in green plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 655–684, 1996.PubMedCrossRefGoogle Scholar
  12. Ivanov, A.G., Morgan, R.M., Gray, G.R., Velitchkova, M.Y., Huner, N.P.A.: Temperature/light dependent development of selective resistance to photoinhibition of photosystem I.-FEBS Lett. 430: 288–292, 1998.PubMedCrossRefGoogle Scholar
  13. Karim, A., Fukamachi, H., Hidaka, T.: Photosynthetic performance of Vigna radiata L. leaves developed at different temperature and irradiance levels.-Plant Sci. 164: 451–458, 2003.CrossRefGoogle Scholar
  14. Krause, G.H.: Photoinhibition of photosynthesis. An evaluation of damaging and protective mechanisms.-Physiol. Plant. 74: 566–574, 1988.CrossRefGoogle Scholar
  15. Krause, G.H., Weis, E.: Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals.-Photosynth. Res. 5: 139–157, 1984.CrossRefGoogle Scholar
  16. Laing, W.A., Greer, D.H., Schnell, T.A.: Photoinhibition of photosynthesis causes a reduction in vegetative growth rates of dwarf bean (Phaseolus vulgaris) plants.-Aust. J. Plant Physiol. 22: 511–520, 1995.CrossRefGoogle Scholar
  17. Lauriano, J.A., Lidon, F.C., Carvalho, C.A., Campos, P.S., do Céu Matos, M.: Drought effects on membrane lipids and photosynthetic activity in different peanut cultivars.-Photosynthetica 38: 7–12, 2000.CrossRefGoogle Scholar
  18. Long, S.P., Humphries, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633–662, 1994.CrossRefGoogle Scholar
  19. Macpherson, A.N., Telfer, A., Barber, J., Truscott, T.G.: Direct detection of singlet oxygen from isolated photosystem II reaction centres.-Biochim. biophys. Acta 1143: 301–309, 1993.CrossRefGoogle Scholar
  20. Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence — a practical guide.-J. exp. Bot. 345: 659–668, 2000.CrossRefGoogle Scholar
  21. Mishra, N.P., Ghanotakis, D.F.: Exposure of a photosystem II complex to chemically generated singlet oxygen results in D1 fragments similar to the ones observed during aerobic photoinhibition.-Biochim. biophys. Acta 1187: 296–300, 1994.CrossRefGoogle Scholar
  22. Mittler, R.: Oxidative stress, antioxidants and stress tolerance.-Trends Plant Sci. 9: 405–410, 2002.CrossRefGoogle Scholar
  23. Nedbal, L., Masojídek, J., Komenda, J., Prášil, O., Šetlík, I.: Three types of photosystem II photoinactivation. 2. Slow processes.-Photosynth. Res. 24: 89–97, 1990.CrossRefGoogle Scholar
  24. Ohad, I., Kyle, D.J., Arntzen, C.J.: Membrane protein damage and repair. II. Removal and replacement of inactivated 32-kilodalton polypeptides in chloroplast membranes.-J. Cell Biol. 99: 481–485, 1984.PubMedCrossRefGoogle Scholar
  25. Powles, S.B., Berry, J.A., Björkman, O.: Interaction between light and chilling temperature on the inhibition of photosynthesis in chilling-sensitive plants.-Plant Cell Environ. 6: 117–123, 1983.CrossRefGoogle Scholar
  26. Scandalios, J.G.: Oxygen stress and superoxide dismutases.-Plant Physiol. 101: 7–12, 1993.PubMedGoogle Scholar
  27. Schansker, G., van Rensen, J.J.S.: Performance of active photosystem II centers in photoinhibited pea leaves.-Photosynth. Res. 62: 175–184, 1999.CrossRefGoogle Scholar
  28. Schreiber, U., Bilger, W., Neubauer, C.: Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis.-In: Schulze, E.-D., Caldwell, M.M. (ed.): Ecophysiology of Photosynthesis. Pp. 49–70. Springer-Verlag, Berlin 1994.Google Scholar
  29. Schuster, G., Timberg, R., Ohad, I.: Turnover of thylakoid photosystem II proteins during photoinhibition of Chlamydomonas reinhardtii.-Eur. J. Biochem. 177: 403–410, 1988.PubMedCrossRefGoogle Scholar
  30. Silva, F.F.: Photochemical Efficiency, Radiation Use Efficiency and Productivity in Bean (Phaseolus vulgaris and Vigna unguiculata) Cultivated in Summer and Winter, in Rio de Janeiro State, Brazil.-MS Thesis. Universidade Estadual do Norte Fluminense, Campos dos Goytacazes 2001.Google Scholar
  31. Tsonev, T., Velikova, V., Georgieva, K., Hyde, P.F., Jones, H.G.: Low temperature enhances photosynthetic down-regulation in French bean (Phaseolus vulgaris L.) plants.-Ann. Bot. 91: 343–352, 2003.PubMedCrossRefGoogle Scholar
  32. Wise, R.R., Naylor, A.W.: Chilling-enhanced photooxidation. The peroxidative destruction of lipids during chilling injury to photosynthesis and ultrastructure.-Plant Physiol. 83: 272–277, 1987a.PubMedGoogle Scholar
  33. Wise, R.R., Naylor, A.W.: Chilling-enhanced photooxidation. Evidence for the role of singlet oxygen and superoxide in the breakdown of pigments and endogenous antioxidants.-Plant Physiol. 83: 278–282, 1987b.PubMedCrossRefGoogle Scholar
  34. Yordanov, I., Tsonev, T., Goltsev, V., Kruleva, L., Velikova, V.: Interactive effect of water deficit and high temperature on photosynthesis in sunflower and maize plants. 1. Changes in parameters of chlorophyll fluorescence induction kinetics and fluorescence quenching.-Photosynthetica 33: 391–402, 1997.Google Scholar

Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  • L. C. S. Ferreira
    • 1
  • R. Bressan-Smith
    • 1
  • T. F. Elias
    • 1
  • F. F. Silva
    • 1
  • L. H. Viana
    • 1
  • J. G. Oliveira
    • 1
  1. 1.Setor de Fisiologia Vegetal, LMGV/CCTAUniversidade Estadual do Norte FluminenseCampos dos Goytacazes, RJBrazil

Personalised recommendations