, Volume 45, Issue 1, pp 1–8 | Cite as

Interactions between photosynthetic pigments in organisms and in model systems

  • D. Frąckowiak
  • B. Smyk


The review summarizes results concerning photosynthetic systems with chlorophylls and carotenoids obtained by means of spectral methods such as polarized radiation, photoacoustic spectroscopy, delayed luminescence, thermal deactivation, and leading to construction of model systems.

Additional key words

carotenoids chlorophylls delayed luminescence excitation energy migration models photoacoustic spectroscopy photosystem 2 reaction centres polarized radiation thermal deactivation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Birks, J.B.: Photophysics of Aromatic Molecules.-John Wiley and Sons, London 1970.Google Scholar
  2. Braslavsky, S.E., Heibel, G.E.: Time resolved photothermal and photoacoustic methods applied in photoinduced processes in solution.-Chem. Rev. 92: 1381–1410, 1992.CrossRefGoogle Scholar
  3. Cegielski, R., Frąckowiak, D., Liang, J.: Circular dichroism spectra of axially oriented Rhodospirillum rubrum cells and their fragments immobilized in polymer films.-Acta phys. polon. 79: 479–491, 1991.Google Scholar
  4. Cogdell, R.J., Frank, H.A.: How carotenoids function in photosynthetic bacteria.-Biochim. biophys. Acta 895: 63–97, 1987.PubMedGoogle Scholar
  5. De Las Rivas, J., Telfer, A., Barber, J.: Two coupled β-carotene molecules protect P680 from photodamage in isolated Photosystem II reaction centres.-Biochim. biophys. Acta 1142: 155–164, 1993.CrossRefGoogle Scholar
  6. Frąckowiak, D.: Joint application of fluorescence and photoacoustic methods in photobiology.-Appl. Fluorescence Technol. 2: 11–14, 1990.Google Scholar
  7. Frąckowiak, D., Bauman, D., Fiksiński, K., Wróbel, D.: Photosynthetic pigments and pigment-protein complexes in anisotropic media.-In: Akoyunoglou, G. (ed.): Photosynthesis I. Pp. 263–272. Balaban Int. Science Services, Philadelphia 1981.Google Scholar
  8. Frąckowiak, D., Bauman, D., Stillman, M.J.: Circular dichroism and magnetic circular dichroism spectra of chlorophylls in nematic liquid crystals. I. Electric and weak magnetic field effects of the dichroism spectra.-Biochim. biophys. Acta 681: 273–285, 1982.CrossRefGoogle Scholar
  9. Frąckowiak, D., Cegielski, R., Abdourakhmanov, I., Zelent, B., Leblanc, R.M: Decay of fluorescence of purple bacteria cells and complexes embedded in polyvinyl alcohol films.-Photosynthetica 26: 563–569, 1992.Google Scholar
  10. Frąckowiak, D., Cegielski, R., Niedbalska, M., Waloszek, A., Więckowski, S.: Thermal deactivation of excitation in pigment molecules in thylakoids.-Photosynthetica 32: 439–453, 1996a.Google Scholar
  11. Frąckowiak, D., Dudkowiak, A., Cegielski, R., Planner, A., Ptak, A., Schultz, C.: Slow paths of pigments deexcitation in reaction centers from the carotenoidless purple bacterium Rhodobacter sphaeroides.-Photosynthetica 32: 105–115, 1996b.Google Scholar
  12. Frąckowiak, D., Dudkowiak, A., Cegielski, R., Planner, A., Schulz, C.: Optical properties of the purple bacterium reaction centers immobilized in polymer film.-Photosynthetica 31: 283–299, 1995.Google Scholar
  13. Frąckowiak, D., Dudkiewicz, J., Grabowski, J., Fiksiński, K., Manikowski, H.: Spectral properties of phycoerythrin.-Photosynthetica 13: 21–28, 1979.Google Scholar
  14. Frąckowiak, D., Dudkowiak, A., Ptak, A., Malak, H., Gryczyński, I., Zelent, B.: Fluorescence lifetimes of oriented green bacteria cells, cell fragments and oriented bacteriochlorophyll c molecules.-J. Photochem. Photobiol. B 44: 231–239, 1998.CrossRefGoogle Scholar
  15. Frąckowiak, D., Dudkowiak, A., Zelent, B., Leblanc, R.M.: Fluorescence anisotropy of cyanobacterial phycobilisomes oriented in PVA films.-J. Fluorescence 1: 225–234, 1992.CrossRefGoogle Scholar
  16. Frąckowiak, D., Goc, J., Waszkowiak, A.: Biophysics in technology and medicine.-Curr. Topics Biophys. 24: 3–13, 2000.Google Scholar
  17. Frąckowiak, D., Grabowski, J., Stachowiak-Hans, Z.: Energy transfer between bile pigments and chlorophyll in vivo.-Photosynthetica 3: 39–44, 1969.Google Scholar
  18. Frąckowiak, D., Hans, E.: Benzoquinone quenching of fluorescence of preirradiated Chlorella.-Photosynthetica 6: 298–302, 1972.Google Scholar
  19. Frąckowiak, D., Hendrich, W., Romanowski, M., Szczepaniak, A., Leblanc, R.M.: Polarized spectra of PS2 particles in PVA films.-In: Biggins, J. (ed.): Progress in Photosynthesis Research. Vol. 1. Pp. 127–130. M. Nijhoff Publ., Dordrecht-Boston-Lancaster 1987.Google Scholar
  20. Frąckowiak, D., Hotchandani, S., Leblanc, R.M.: Photoacoustic spectra of phycobiliproteins and chlorophyllin in isotropic and anisotropic polyvinyl alcohol films.-Photochem. Photobiol. 42: 559–565, 1985.Google Scholar
  21. Frąckowiak, D., Manikowski, H.: Effect of dark storage on absorption and fluorescence spectra of Rhodospirillum rubrum cells and fragments.-Photosynthetica 7: 275–281, 1973.Google Scholar
  22. Frąckowiak, D., Miedziejko, E., Surma, S.: Energy transfer between chloroplast dyes.-In: Photochemistry and Photobiology in Plant Physiology. Book of Abstracts. Pp. 17–19. Hvar 1967.Google Scholar
  23. Frąckowiak, D., Minuro, M., Yamazaki, I., Tamai, M., Fujita, Y.: Polarized time-resolved fluorescence spectra of phycobilisomes isolated from Tolypothrix tenuis embedded in poly(vinyl alcohol) film.-Photochem. Photobiol. 50: 563–570, 1989.Google Scholar
  24. Frąckowiak, D., Naser, N.S.: Photopotential generation in model systems and in photosynthetic organisms.-Photosynthetica 33: 371–383, 1997.Google Scholar
  25. Frąckowiak, D., Ptak, A.: Radiative and thermal deexcitation of photosynthetic pigments in organisms and model systems.-Photosynthetica 30: 553–566, 1994.Google Scholar
  26. Frąckowiak, D., Ptak, A.: Photoelectric properties of the solutions of dyes, biological pigments and pigment-protein complexes located in electrochemical cell.-Curr. Topics Electrochem. 10: 179–189, 2004.Google Scholar
  27. Frąckowiak, D., Salamon, Z.: The protective action of carotenoids on fluorescence of chlorophyll b.-Photochem. Photobiol. 11: 559–563, 1970.PubMedGoogle Scholar
  28. Frąckowiak, D., Stillman, M.J.: Magnetic circular dichroism study of a chlorophyll complex ordered by a nematic liquid crystal.-Acta phys. polon. A 67: 829–833, 1984.Google Scholar
  29. Frąckowiak, D., Więckowski, S., Waloszek, A., Planner, A., Ptak, A., Hanyż I., Goc, J.: Chlorophyll-carotenoids interactions in model system and in organisms investigated by photoacoustic spectroscopy.-Curr. Topics Biophys. 19: 71–79, 1997.Google Scholar
  30. Frąckowiak, D., Wróbel, D.: Energy transfer between chlorophyll c and chlorophyll a.-Biophys. Chem. 1: 125–129, 1973.PubMedCrossRefGoogle Scholar
  31. Frąckowiak, D., Wróbel, D., Dudkowiak, A.: Application of the polarized light photoacoustic in photosynthesis research.-Trends Photochem. Photobiol. 2: 411–420, 1991.Google Scholar
  32. Frąckowiak, D., Zelent, B., Malak, H., Cegielski, R., Goc, J., Niedbalska, M., Ptak, A.: Interaction between chlorophyll a and β-carotene in nematic liquid crystals.-Biophys. Chem. 54: 95–107, 1995.PubMedCrossRefGoogle Scholar
  33. Giese, A.C.: Photophysiology. Vol. 1.-Academic Press, New York-London 1977.Google Scholar
  34. Govindjee, Govindjee, R.: Introduction to photosynthesis.-In: Govindjee (ed.): Bioenergetics of Photosynthesis. Pp. 1–50. Academic Press, New York-San Francisco-London 1975.Google Scholar
  35. Gurinovich, G.P., Losev, A.P., Zen’kevich, E.I.: [Primary physical processes in photosynthesis.]-In: Shlyk, A.A. (ed.): Khlorofill. Pp. 74–84. Nauka Tekhnika, Minsk 1974. [In Russ.]Google Scholar
  36. Hanyż, I., Pieńkowska, H., Dudkowiak, A., Frąckowiak, D.: The photochemical stability of oil from Evening Primrose seeds.-Dyes Pigments 70: 177–184, 2006.CrossRefGoogle Scholar
  37. Kowalczyk, A., Malak, H., Zelent, B., Dudkowiak, A., Frąckowiak, D.: Analysis of the background (ns) fluorescence decay of green bacteria cells immobilized in a polymer.-Photosynthetica 32: 613–616, 1996b.Google Scholar
  38. Kowalczyk, A., Zelent, B., Malak, H., Planner, A., Sanocka, S., Frąckowiak, D.: Analysis of fluorescence decays of chlorophyll a and β-carotene in nematic liquid crystal.-Spectroscopy Lett. 29: 367–378, 1996a.Google Scholar
  39. Krausz, E., Hughes, J.L., Smith, P., Pace, R., Årsköld, S.P.: Oxygen-evolving Photosystem II core complexes: a new paradigm based on the spectral identification of the charge-separating state, the primary acceptor and assignment of low-temperature fluorescence.-Photochem. photobiol. Sci. 4: 744–753, 2005.PubMedCrossRefGoogle Scholar
  40. Kruse, O., Rupprecht, J., Mussgnug, J.H., Dismukes, G.C., Hankamer, B.: Photosynthesis a blueprint for solar energy and biohydrogen production.-Photochem. photophys. Sci. 4: 957–969, 2005.CrossRefGoogle Scholar
  41. Lorrain, L., Frąckowiak, D., Romanowski, M., Leblanc, R.M.: Effect of stretching of chloroplasts and thylakoids embedded in polymer films.-Photosynthetica 21: 43–50, 1987.Google Scholar
  42. Łukasiewicz, J., Dudkowiak, A., Pieńkowska, H., Frąckowiak, D.: Radiative and nonradiative deexcitation of pigments from natural oils.-J. Phys. IV France: in press, 2006.Google Scholar
  43. Martyński, T., Frąckowiak, D., Miyake, J., Dudkowiak, A., Piechowiak, A.: The orientation of bacteriochlorophyll c in green bacteria cells and cell fragments.-J. Photochem. Photobiol. 42: 57–66, 1998.CrossRefGoogle Scholar
  44. Mathis, P., Schenck, C.: The functions of carotenoids in photosynthesis.-In: Britton, G., Goodwin, T.W. (ed.): Carotenoid Chemistry and Biochemistry. Pp. 339–351. Pergamon Press, Oxford-New York-Toronto-Sydney-Paris-Frankfurt 1982.Google Scholar
  45. Naser, N.S., Planner, A., Frąckowiak, D.: Photoelectrochemical cell with dye molecules oriented in nematic liquid crystal.-Acta phys. polon. A 92: 535–542, 1997.Google Scholar
  46. Ouzafe, M., Poulet, P., Chambron, J.: Photoacoustic detection of triplet state and singlet oxygen in highly absorbing samples.-Photochem. Photobiol. 55: 491–503, 1992.PubMedGoogle Scholar
  47. Pieńkowska, H., Dudkowiak, A., Muszyński, D., Frąckowiak, D.: The efficiency of the generation of photochemically active triplet states in oils containing various amounts of natural pigments.-Dyes Pigments 64: 109–116, 2005.CrossRefGoogle Scholar
  48. Pieńkowska, H., Smyk, B., Zadernowski, R.: [Spectral properties of biooils obtained from seeds of selected alternative plants.]-Zesz. problem. Postępów Nauk roln. 468: 403–411, 1999. [In Polish.]Google Scholar
  49. Planner, A., Goc, J., Dudkowiak, A., Frąckowiak, D., Miyake, J.: The influence of the presence of lipid on the aggregation of 8,12-diethylfarnesyl bacteriochlorophyll c located in adsorbed layers and monolayers.-J. Photochem. Photobiol. B 39: 73–80, 1997.PubMedCrossRefGoogle Scholar
  50. Ptak, A., Chrzumnicka, E., Dudkowiak, A., Frąckowiak, D.: Electrochemical cell with bacteriochlorophyll c and chlorophylls a and b in nematic liquid crystal.-J. Photochem. Photobiol. A 98: 159–163, 1996.CrossRefGoogle Scholar
  51. Ptak, A., Chrzumnicka, E., Planner, A., Frąckowiak, D.: Photoelectric properties of chlorophyll and carotene solutions in nematic liquid crystal located between semiconducting electrodes.-Biophys. Chem. 65: 165–170, 1997a.PubMedCrossRefGoogle Scholar
  52. Ptak, A., Der, A., Toth-Boconadi, R., Naser, N.S., Frąckowiak, D.: Photocurrent kinetics (in the microsecond time range) of chlorophyll a, chlorophyll b and stilbazolium merocyanine solutions in a nematic liquid crystal located in an electrochemical cell.-J. Photochem. Photobiol. A 104: 133–139, 1997b.CrossRefGoogle Scholar
  53. Ptak, A., Dudkowiak, A., Frąckowiak, D.: Photoelectrical properties of green bacteria cells and cell fragments located in electrochemical cell.-J. Photochem. Photobiol. A 115: 63–68, 1998.CrossRefGoogle Scholar
  54. Rosenzweig, A.: Photoacoustics and Photoacoustic Spectroscopy.-John Wiley and Sons, New York-Chichester-Brisbane-Toronto 1980.Google Scholar
  55. Skibiński, A., Frąckowiak, D., Planner, A.: Delayed luminescence of cyanobacterium Tolypothrix tenuis adapted to green and red radiation.-Photosynthetica 28: 57–61, 1993.Google Scholar
  56. Telfer, A.: What is β-carotene doing in the photosystem II reaction centre?-Phil. Trans. roy. Soc. London B 357: 1431–1440, 2002.PubMedCrossRefGoogle Scholar
  57. Telfer, A.: Too much light? How β-carotene protects the photosystem II reaction centre.-Photochem. photobiol. Sci. 4: 950–956, 2005.PubMedCrossRefGoogle Scholar
  58. Telfer, A., Barber, J.: Role of carotenoid bound to the photosystem II reaction centre.-In: Mathis, P. (ed.): Photosynthesis: From Light to the Biosphere. Vol. IV. Pp. 15–20. Kluwer Academic Publ., Dordrecht-Boston-London 1995.Google Scholar
  59. Telfer, A., Bishop, S.M., Philips, D., Barber, J.: Isolated photosynthetic reaction center of photosystem II as a sensitizer for the formation of singlet oxygen. Detection and quantum yield determination using a chemical trapping technique.-J. biol. Chem. 269: 13244–13253, 1994.Google Scholar
  60. Telfer, A., De Las Rivas, J., Barber, J.: β-carotene within the isolated Photosystem II reaction centre: photooxidation and irreversible bleaching of this chromophore by oxidised P680.-Biochim. biophys. Acta 1060: 106–114, 1991.CrossRefGoogle Scholar
  61. Telfer, A., Dhami, S., Bishop, S.M., Phillips, D., Barber, J.: β-carotene quenches singlet oxygen formed by isolated photosystem II reaction centers.-Biochemistry 33: 14469–14474, 1994.Google Scholar
  62. Telfer, A., Frolov, D., Barber, J., Robert, B., Pascal, A.: Oxidation of the two β-carotene molecules in the photosystem II reaction center.-Biochemistry 42: 1008–1115, 2003.PubMedCrossRefGoogle Scholar
  63. Tien, H.T., Karvaly, B.: Ultrathin barriers for solar energy conversion.-In: Bolton, J.B. (ed.): Solar Power and Fuels. P. 167. Academic Press, New York-San Francisco-London 1977.Google Scholar

Copyright information

© Institute of Experimental Botany, ASCR 2007

Authors and Affiliations

  • D. Frąckowiak
    • 1
  • B. Smyk
    • 2
  1. 1.Institute of PhysicsPoznań University of TechnologyPoznańPoland
  2. 2.Department of Physics and BiophysicsUniversity of Warmia and Mazury in OlsztynOlsztynPoland

Personalised recommendations