Pharmaceutical Research

, Volume 34, Issue 3, pp 591–598 | Cite as

Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity

  • Karina A. Crespo
  • José L. Baronetti
  • Melisa A. Quinteros
  • Paulina L. Páez
  • María G. Paraje
Research Paper



The use of microorganisms for the synthesis of nanoparticles (NPs) is relatively new in basic research and technology areas.


This work was conducted to optimized the biosynthesis of iron NPs intra- and extracellular by Escherichia coli or Pseudomonas aeruginosa and to evaluate their anticoagulant activity.

Study Design/Methods

The structures and properties of the iron NPs were investigated by Ultraviolet–visible (UV-vis) spectroscopy, Zeta potential, Dynamic light scattering (DLS), Field emission scanning electron microscope (FESEM)/ Energy dispersive X-ray (EDX) and transmission electron microscopy (TEM). Anticoagulant activity was determined by conducting trials of Thrombin Time (TT), Activated Partial Prothrombin Time (APTT) and Prothrombin Time (PT).


UV-vis spectrum of the aqueous medium containing iron NPs showed a peak at 275 nm. The forming of iron NPs was confirmed by FESEM/ EDX, and TEM. The morphology was spherical shapes mostly with low polydispersity and the average particle diameter was 23 ± 1 nm. Iron NPs showed anticoagulant activity by the activation of extrinsic pathway.


The eco-friendly process of biosynthesis of iron NPs employing prokaryotic microorganisms presents a good anticoagulant activity. This could be explored as promising candidates for a variety of biomedical and pharmaceutical applications.


anticoagulant activity Escherichia coli iron nanoparticles microbial biosynthesis Pseudomonas aeruginosa 



Activated protombin time test


Dynamic light scattering


Energy dispersive X-ray


Field emission scanning electron microscope




Protombin time


Surface plasmon resonance


Transmission electron microscopy


Trypticase soy broth


Thrombin time





PL Páez and MG Paraje are members of the Research Career of CONICET. KA Crespo is a postdoctoral fellow of CONICET. MA Quinteros is PhD fellow of CONICET. We are also very grateful to Wiener Lab SAIC. This work was supported by the following Grants: SECyT, FONCyT and CONICET.

Compliance with Ethical Standards

Conflicts of Interest

The authors declare that they have not conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Karina A. Crespo
    • 1
  • José L. Baronetti
    • 1
    • 2
  • Melisa A. Quinteros
    • 1
    • 4
  • Paulina L. Páez
    • 3
    • 4
  • María G. Paraje
    • 1
    • 2
  1. 1.Instituto Multidisciplinario de Biología Vegetal (IMBIV) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos AiresArgentina
  2. 2.Cátedra de Microbiología, Facultad de Ciencias Exactas Físicas y NaturalesUniversidad Nacional de Córdoba (UNC)CórdobaArgentina
  3. 3.Unidad de Tecnología Farmacéutica (UNITEFA) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)Buenos AiresArgentina
  4. 4.Departamento de Farmacia, Facultad de Ciencias QuímicasUniversidad Nacional de Córdoba (UNC)CórdobaArgentina

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