Intra- and Extracellular Biosynthesis and Characterization of Iron Nanoparticles from Prokaryotic Microorganisms with Anticoagulant Activity
The use of microorganisms for the synthesis of nanoparticles (NPs) is relatively new in basic research and technology areas.
This work was conducted to optimized the biosynthesis of iron NPs intra- and extracellular by Escherichia coli or Pseudomonas aeruginosa and to evaluate their anticoagulant activity.
The structures and properties of the iron NPs were investigated by Ultraviolet–visible (UV-vis) spectroscopy, Zeta potential, Dynamic light scattering (DLS), Field emission scanning electron microscope (FESEM)/ Energy dispersive X-ray (EDX) and transmission electron microscopy (TEM). Anticoagulant activity was determined by conducting trials of Thrombin Time (TT), Activated Partial Prothrombin Time (APTT) and Prothrombin Time (PT).
UV-vis spectrum of the aqueous medium containing iron NPs showed a peak at 275 nm. The forming of iron NPs was confirmed by FESEM/ EDX, and TEM. The morphology was spherical shapes mostly with low polydispersity and the average particle diameter was 23 ± 1 nm. Iron NPs showed anticoagulant activity by the activation of extrinsic pathway.
The eco-friendly process of biosynthesis of iron NPs employing prokaryotic microorganisms presents a good anticoagulant activity. This could be explored as promising candidates for a variety of biomedical and pharmaceutical applications.
KEY WORDSanticoagulant activity Escherichia coli iron nanoparticles microbial biosynthesis Pseudomonas aeruginosa
Activated protombin time test
Dynamic light scattering
Energy dispersive X-ray
Field emission scanning electron microscope
Surface plasmon resonance
Transmission electron microscopy
Trypticase soy broth
ACKNOWLEDGMENTS AND DISCLOSURES
PL Páez and MG Paraje are members of the Research Career of CONICET. KA Crespo is a postdoctoral fellow of CONICET. MA Quinteros is PhD fellow of CONICET. We are also very grateful to Wiener Lab SAIC. This work was supported by the following Grants: SECyT, FONCyT and CONICET.
Compliance with Ethical Standards
Conflicts of Interest
The authors declare that they have not conflict of interest.
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