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Preparation, Physicochemical Characterization and Anti-fungal Evaluation of Nystatin-Loaded PLGA-Glucosamine Nanoparticles

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ABSTRACT

Purpose

Nystatin loaded PLGA and PLGA-Glucosamine nanoparticles were formulated. PLGA were functionalized with Glucosamine (PLGA-GlcN) to enhance the adhesion of nanoparticles to Candida Albicans (C.albicans) cell walls.

Method

Quasi-emulsion solvent diffusion method was employed using PLGA and PLGA-GlcN with various drug–polymer ratios for the preparation of nanoparticles. The nanoparticles were evaluated for size, zeta potential, polydispersity index, drug crystallinity, loading efficiency and release properties. DSC, SEM, XRPD, 1H-NMR, and FT-IR were performed to analyze the physicochemical properties of the nanoparticles. Antifungal activity of the nanoparticles was evaluated by determination of MICs against C.albicans.

Results

The spectra of 1H-NMR and FT-IR analysis ensured GlcN functionalization on PLGA nanoparticles. SEM characterization confirmed that particles were in the nanosize range and the particle size for PLGA and PLGA-GlcN nanoparticles were in the range of 108.63 ± 4.5 to 168.8 ± 5.65 nm and 208.76 ± 16.85 nm, respectively. DSC and XRPD analysis ensured reduction of the drug crystallinity in the nanoparticles. PLGA-GlcN nanoparticles exhibit higher antifungal activity than PLGA nanoparticles.

Conclusion

PLGA-GlcN nanoparticles showed more antifungal activity with appropriate physicochemical properties than pure Nystatin and PLGA nanoparticles.

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Abbreviations

C. albicans:

Candida Albicans

D2O:

Deuterium oxide

DCM:

Dichloromethane

DMAP:

4-Dimethylaminopyridine

DMSO:

Dimethylsulfoxide

DSC:

Differential scanning calorimetric

FT-IR:

Fourier transform infrared spectroscopy

GlcN:

Glucosamine

H-NMR:

Proton nuclear magnetic resonance

HPLC:

High performance liquid chromatography

KBr:

Potassium Bromide

MIC:

Minimum inhibitory concentration

PE:

Prediction error

PEG:

Polyethylene glycol

PLGA:

Poly lactic-co-glycolic acid

PLGA-GlcN:

PLGA functionalized with Glucosamine

PVA:

Polyvinyl alcohol

RSQ:

Squared correlation coefficient

SEM:

Scanning Electron Microscope

XRPD:

Powder X-Ray Diffractometry

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Author information

Authors and Affiliations

  1. Novel Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences,, Kermanshah, Iran

    Ghobad Mohammadi, Ali Fattahi & Pardis Mohammadi

  2. Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Ghobad Mohammadi

  3. Student Research Committee, Kermanshah University of Medical Sciences,, Kermanshah, Iran

    Amineh Shakeri

  4. Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Ali Mikaeili

  5. Department of Medicinal Chemistry, Faculty of Pharmacy, Kermanshah University of Medical Sciences,, Kermanshah, Iran

    Alireza Aliabadi

  6. Faculty of Pharmacy, Tabriz University of Medical Sciences,, Tabriz, Iran

    Khosro Adibkia

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  1. Ghobad Mohammadi
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Correspondence to Ghobad Mohammadi.

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Mohammadi, G., Shakeri, A., Fattahi, A. et al. Preparation, Physicochemical Characterization and Anti-fungal Evaluation of Nystatin-Loaded PLGA-Glucosamine Nanoparticles. Pharm Res 34, 301–309 (2017). https://doi.org/10.1007/s11095-016-2062-6

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  • DOI: https://doi.org/10.1007/s11095-016-2062-6

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