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Pharmaceutical Research

, Volume 31, Issue 10, pp 2868–2875 | Cite as

Development of Liposomal Nanoconstructs Targeting P-selectin (CD62P)-expressing Cells by Using A Sulfated Derivative of Sialic Acid

  • Saotomo Itoh
  • Kumi Kawano
  • Kana Takeshita
  • Yoshie Maitani
  • Tsutomu Tsuji
Research Paper
  • 258 Downloads

Abstract

Purpose

NMSO3, a sulfated derivative of sialic acid, is a specific inhibitor for P-selectin (CD62P)-mediated cell adhesion. We attempted to apply liposomes modified with NMSO3 for selective targeting of activated platelets.

Methods

The binding of fluorescently labeled NMSO3-containing liposomes (NMSO3-liposomes) to CHO cells expressing P-selectin (CHO-P cells) and activated platelets were examined. The distribution of NMSO3-liposomes incorporated into the cells was observed by fluorescence microscopy.

Results

The binding assay revealed that NMSO3-liposomes specifically bound to immobilized P-selectin and CHO-P cells in a dose-dependent manner. The binding of NMSO3-liposomes to CHO-P cells was much stronger than that to the parental CHO-K1 cells. Fluorescence microscopic observation showed that NMSO3-liposomes were incorporated into CHO-P cells after the binding and distributed throughout the cytoplasm of the cell. NMSO3-liposomes bound more strongly to thrombin-activated platelets than to resting platelets, as assessed by flow cytometry.

Conclusions

These results suggest that NMSO3-liposomes can be applied for selective drug delivery to activated platelets.

KEY WORDS

activated platelet drug delivery liposome P-selectin sulfated sialyl lipid 

Abbreviations

PBS

Phosphate buffered saline

PRP

Platelet-rich plasma

sLeX

Sialyl Lewis X

Notes

Acknowledgments and Disclosure

We are grateful to Dr. Masaaki Kurihara (Kurihara Clinic, Tokyo, Japan) for his helpful discussion of the study. We also would like to thank Mr. Y. Hirakouchi and Mr. H. Maruyama (Hoshi University School of Pharmacy and Pharmaceutical Sciences) for their technical assistance. This work was supported in part by the Ministry of Education, Culture, Sports, Science and Technology of Japan, and by the Open Research Center Project.

The authors have no potential conflicts of interest to disclose.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Saotomo Itoh
    • 1
  • Kumi Kawano
    • 2
  • Kana Takeshita
    • 1
  • Yoshie Maitani
    • 2
  • Tsutomu Tsuji
    • 1
  1. 1.Department of MicrobiologyHoshi University School of Pharmacy and Pharmaceutical SciencesShinagawa-kuJapan
  2. 2.Institute of Medicinal ChemistryHoshi UniversityShinagawa-kuJapan

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