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Pharmaceutical Research

, 27:200 | Cite as

Preclinical Evaluation of Linear HPMA-Doxorubicin Conjugates with pH-Sensitive Drug Release: Efficacy, Safety, and Immunomodulating Activity in Murine Model

  • Milada Sirova
  • Tomas Mrkvan
  • Tomas Etrych
  • Petr Chytil
  • Pavel Rossmann
  • Marketa Ibrahimova
  • Lubomir Kovar
  • Karel Ulbrich
  • Blanka Rihova
Research Paper

ABSTRACT

Purpose

In vivo efficacy and safety of HPMA-based copolymers armed with doxorubicin via a spacer containing pH-sensitive linkage that can be prepared within a broad range of attached drug contents (1) was tested in murine tumor models.

Methods

Mice bearing T cell lymphoma EL4 or B cell lymphoma 38C13 were treated with a single dose of the conjugate (15, 25, and 75 mg Dox eq./kg i.v.) in a therapeutic regime. Anti-tumor resistance of the cured animals was proved by a second challenge with a lethal dose of tumor cells without additional treatment.

Results

The content of drug bound to the polymer is an important parameter in relation to the conjugate therapeutic efficacy. The best anti-tumor effects were produced by conjugates with 10 – 13 wt% of bound doxorubicin. Free doxorubicin up to 4.6% relative to total drug content had no impact on the treatment efficacy and acute toxicity. The conjugates induced a complete cure of mice and regular treatment-dependent development of specific anti-tumor resistance. No myelosuppression or organ damage was observed.

Conclusions

A well-defined HPMA copolymer-doxorubicin conjugate with pH-sensitive drug release is a good candidate for clinical trials as it has remarkable anti-tumor efficacy and a favorable safety profile.

KEY WORDS

doxorubicin immunomodulation murine lymphoma N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer conjugate tumor resistance 

ABBREVIATIONS

B/6

C57BL/6

CRT

calreticulin

Dox

doxorubicin

HMGB1

high mobility group box-1

HPMA

N-(2-hydroxypropyl)methacrylamide

PBS

phosphate buffered saline

Notes

ACKNOWLEDGEMENTS

The work was supported by grant KAN 200200651, Premium Academiae, and Institutional Research Concept AV0Z50200510. Authors appreciate the funding support from pharmaceutical company Zentiva, k.s. (Czech Republic), and thank Mrs. Helena Mišurcová and Ms. Pavlína Jungrová for excellent technical assistance.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Milada Sirova
    • 1
  • Tomas Mrkvan
    • 1
  • Tomas Etrych
    • 2
  • Petr Chytil
    • 2
  • Pavel Rossmann
    • 1
  • Marketa Ibrahimova
    • 1
  • Lubomir Kovar
    • 1
  • Karel Ulbrich
    • 2
  • Blanka Rihova
    • 1
  1. 1.Laboratory of Tumor Immunology, Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPrague 4Czech Republic
  2. 2.Department of Biomedicinal Polymers, Institute of Macromolecular ChemistryAcademy of Sciences of the Czech RepublicPragueCzech Republic

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