Microneedle Arrays Allow Lower Microbial Penetration Than Hypodermic Needles In Vitro
- 693 Downloads
In this study we determined, for the first time, the ability of microorganisms to traverse microneedle-induced holes using two different in vitro models.
When employing Silescol® membranes, the numbers of Candida albicans, Pseudomonas aeruginosa and Staphylococcus epidermidis crossing the membranes were an order of magnitude lower when the membranes were punctured by microneedles rather than a 21G hypodermic needle. Apart from the movement of C. albicans across hypodermic needle-punctured membranes, where 40.2% of the microbial load on control membranes permeated the barrier over 24 h, the numbers of permeating microorganisms was less than 5% of the original microbial load on control membranes. Experiments employing excised porcine skin and radiolabelled microorganisms showed that the numbers of microorganisms penetrating skin beyond the stratum corneum were approximately an order of magnitude greater than the numbers crossing Silescol® membranes in the corresponding experiments. Approximately 103 cfu of each microorganism adhered to hypodermic needles during insertion. The numbers of microorganisms adhering to MN arrays were an order of magnitude higher in each case.
We have shown here that microneedle puncture resulted in significantly less microbial penetration than did hypodermic needle puncture and that no microorganisms crossed the viable epidermis in microneedle—punctured skin, in contrast to needle-punctured skin. Given the antimicrobial properties of skin, it is, therefore, likely that application of microneedle arrays to skin in an appropriate manner would not cause either local or systemic infection in normal circumstances in immune-competent patients. In supporting widespread clinical use of microneedle-based delivery systems, appropriate animal studies are now needed to conclusively demonstrate this in vivo. Safety in patients will be enhanced by aseptic or sterile manufacture and by fabricating microneedles from self-disabling materials (e.g. dissolving or biodegradable polymers) to prevent inappropriate or accidental reuse.
Key Wordsinfection microneedle arrays microorganisms stratum corneum
This work was supported by BBSRC grant number: BB/E020534/1 and the Science Foundation Ireland Tyndall National Access Programme project number NAP 156.
- 6.Cormier M, Johnson B, Ameri M, Nyam K, Libiran L, Zhang DD. Transdermal delivery of desmopressin using a coated microneedle array patch system. J Cont Rel. 2004;97:503–11.Google Scholar
- 20.Seal DV, Hay RJ, Middleton KR. Skin and wound infection: investigation and treatment in practice. London: Informa Healthcare; 2000. p. 5–8.Google Scholar
- 21.www.who.int/en. Accessed 16th April 2009.
- 23.Del Mar CB, Glasziou PP, Spinks AB, Sanders SL. Is isopropyl alcohol swabbing before injection really necessary? Med J Aust. 2001;175:341–2.Google Scholar