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Pharmaceutical Research

, Volume 22, Issue 6, pp 972–980 | Cite as

N4,N9-Dioleoyl Spermine Is a Novel Nonviral Lipopolyamine Vector for Plasmid DNA Formulation

  • Osama A. A. Ahmed
  • Noppadon Adjimatera
  • Charareh Pourzand
  • Ian S. Blagbrough
Research Paper

Purpose

To study the effect of synthesized N4,N9-dioleoyl spermine on DNA condensation and then measure its transfection efficiency in cell culture.

Methods

The lipopolyamine was synthesized from the naturally occurring polyamine spermine. The ability of this novel compound to condense DNA was studied using ethidium bromide fluorescence quenching and light scattering assays. Transfection efficiency was studied in primary skin cells (FEK4) and in an immortalized cancer cell line (HtTA), and compared with the commercially available transfection formulations Lipofectin and Lipofectamine.

Results

The synthesized N4,N9-dioleoyl spermine formula is efficient at condensing calf thymus and circular plasmid DNA and effectively transfects both primary skin cells and cancer cell lines at low charge ratios of (+/− ammonium/phosphate) 2.5.

Conclusions

N4,N9-Dioleoyl spermine condenses DNA and achieves high transfection levels in cultured cells.

Key words

FEK4 gene delivery lipopolyamine N4,N9-dioleoyl spermine transfection 

Abbreviations

DCC

dicyclohexylcarbodiimide

DOGS

dioctadecylamidoglycylspermine

DOSPER

1,3-dioleoyloxy-2-(6-carboxyspermine

EGFP

enhanced green fluorescent protein

EMEM

Earle’s minimal essential medium

EthBr

ethidium bromide

FCS

fetal calf serum

HOBt

hydroxybenzotriazole

NPC

nuclear pore complex

PEI

polyethylenimine

PLL

poly-l-lysine

Notes

Acknowledgments

We acknowledge the financial support of the Egyptian Government studentship to O.A.A. A. and Universities U.K. for an ORS award to N. A. We are grateful to Prof R.M. Tyrrell (University of Bath) for FEK4 and HtTA cell lines.

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Osama A. A. Ahmed
    • 1
  • Noppadon Adjimatera
    • 1
  • Charareh Pourzand
    • 1
  • Ian S. Blagbrough
    • 1
  1. 1.Department of Pharmacy and PharmacologyUniversity of BathBathUnited Kingdom

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