Development of a Spectrophotometric Method for the Determination of Florfenicol in Eudragit Nanocapsules

The objective of this work was to develop a simple spectrophotometric method for determining antibiotic florfenicol in Eudragit nanocapsules. Regression curve of the method is linear in a concentration range of 80 – 480 μg/mL (correlation coefficient, 0.9998) at a selected wavelength of 270 nm. The limits of detection and quantification are 0.4 μg/mL and 1.2 μg/mL, respectively. The developed method demonstrated good inter-day and intra-day precision with RSD < 2. The percentage recovery of florfenicol was within 99.56 – 99.90%. In conclusion, the proposed method is specific, reproducible, accurate, and robust for the determination of florfenicol in Eudragit nanocapsules with detection at 270 nm in 95% confidence interval.

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References

  1. 1.

    G. Kee, W. Calvin, P. Timothy, et al., Can. Vet. J., 40(3), 179 – 184 (1999).

    Google Scholar 

  2. 2.

    V. Aslan, M. Maden, O. Erganis, et al., Vet. Quart., 24(1), 35 – 39 (2002).

    CAS  Article  Google Scholar 

  3. 3.

    L. Gutiérrez, D. Vargas, L. Ocampo, et al., J. Anim. Sci., 89(9), 2926 – 2931 (2011).

    Article  Google Scholar 

  4. 4.

    S. Higuera-Llantén, F. Vásquez-Ponce, B. Barrientos-Espinoza, et al., PLoS One, 13(9), (2018).

  5. 5.

    Z. Xuemei, S. Yu, S. C. Xinxin, et al., Int. Inmunopharmacol., 8(7), 982 – 988 (2008).

    Article  Google Scholar 

  6. 6.

    J. M. Shah, T. A. Qureshi, T. Shah, et al., Vet. World, 9(10), 1135–1140 (2016).

    CAS  Article  Google Scholar 

  7. 7.

    C. Orelle, S. Carlson, B. Kaushal, et al., Antimicrob. Agents Chemother., 57(12), 5994–6004 (2013).

    CAS  Article  Google Scholar 

  8. 8.

    H. Dongfang, H. Ziqiang, L. Chunling, et al., Immunopharmacol. Immunotoxicol., 38(6), 472 – 485 (2016).

    Article  Google Scholar 

  9. 9.

    D. Hu, S. Cao, G. Zhang, et al., Sci. Rep., 7(1), (2017).

  10. 10.

    N. Batrawi, H. Naseef, and F. Al-Rimawi, J. Anal. Meth. Chem. (2017).

  11. 11.

    L. Jiong, G. Jinyan, Y. Haina, et al., J. Chromatogr. Sci. (2020).

  12. 12.

    R. E. Gavilán, C. Nebot, E. Patyra, et al., Antibiotics, 8(2), 59 (2019).

    Article  Google Scholar 

  13. 13.

    B. T. Veach, R. Anglin, T. K. Mudalige, et al., J. AOAC Int., 101(3), 897–904 (2018).

    Article  Google Scholar 

  14. 14.

    E. Mohammed, S. Shaza, G. Elrasheed, et al., Chem. Sci. Trans., 5(4), 1063 – 1067 (2016).

    Google Scholar 

  15. 15.

    M. Elimam, S. Shantier, E. Gadkariem, et al., Int. J. Pharm. Pharmaceut. Sci., 8(5), 347 – 349 (2016).

    CAS  Google Scholar 

  16. 16.

    C. Rome, M. Keramidas, F. Fraipont, et al., Rev. Maladies Respir., 25(9), 1175 (2008).

    Article  Google Scholar 

  17. 17.

    H. Fessi, F. Puisieux, J. Devissaguet, et al., Int. J. Pharm., 55(1), R1 – R4. (1989).

    CAS  Article  Google Scholar 

Download references

Acknowledgments

Dr. Serrano Castañeda wants to acknowledge DGAPA and BASF México. Dr. Escobar-Chávez wants to acknowledge PAPIME PE 201420 an Cátedra PIAPI 2015. This work was supported by UNAM-PAPIIT CG 100220.

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The authors declare that they have no conflict of interest.

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Correspondence to José Juan Escobar-Chávez.

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Castañeda, P.S., Olvera, L.G., Bernad, M.J.B. et al. Development of a Spectrophotometric Method for the Determination of Florfenicol in Eudragit Nanocapsules. Pharm Chem J 54, 1181–1185 (2021). https://doi.org/10.1007/s11094-021-02340-0

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Keywords

  • florfenicol
  • Eudragit nanocapsules
  • spectrophotometric determination