Study on Fundamental Process of Codonopsis pilosula Desulfurization and In Vitro Antibacterial Activity of its Polysaccharide Extract on Escherichia coli
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The present study was aimed to optimize the conditions of Codonopsis pilosula desulfurization using orthogonal experiment analysis with four independent variables including desulfurization temperature, time, ultrasonic power, and ratio of ethanol to raw material. Aspects of the antibacterial activity of polysaccharides extracted from sulfur-fumigated C. pilosula and after desulfurization by the agar-well diffusion method against Escherichia coli have also been studied. The results showed that the optimum extraction conditions were as follows: desulfurization temperature, 45°C; desulfurization time, 50 min; ultrasonic power, 700 W; and ethanol to raw material ratio, 10 : 1. Under these conditions, the desulfurization rate was 55.4%. The antibacterial activity of polysaccharides extracted from desulfurized C. pilosula was higher than that of polysaccharides extracted from sulfur-fumigated herbs, with the MIC values of 35 and 70 mg/mL, respectively.
Keywordsdesulfurization antibacterial activity polysaccharides Codonopsis pilosula
This research was financially supported by the Shanxi province science and technology key project of China (20130313015-2). It was also supported by the National Natural Science Foundation of China (31401319), the Program for the Top Young Academic Leaders of Higher Learning Institutions of Shanxi of China (20151006), and Shanxi Province Science Foundation for Youths (2015021146). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Conceived and designed the experiments: WYZ. Performed the experiments, contributed reagents/materials/analysis tools, and wrote the paper: WYZ, ERW.
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