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Pharmaceutical Chemistry Journal

, Volume 49, Issue 1, pp 59–64 | Cite as

Development and Validation of a Method for Assay of Residual Organic Solvents in Kemantane Substance by Gas Liquid Chromatography

  • A. V. Tolkacheva
  • L. N. Grushevskaya
  • N. I. Avdyunina
  • B. M. Pyatin
  • V. I. Prokof’eva
  • L. M. Gaevaya
  • N. M. Zaitseva
STRUCTURE OF CHEMICAL COMPOUNDS, METHODS OF ANALYSIS AND PROCESS CONTROL

The aim of the present work was to develop and validate an assay for residual organic solvents in kemantane substance (5-hydroxyadamantan-2-one) by gas liquid chromatography (GLC-FID). Experiments were performed using a gas chromatograph with a flame ionization detector and an autosampler. Chromatographic separation was performed on a VF-624ms quartz capillary column of length 60 m and diameter 0.32 mm, with an applied immobile phase 1.80 μm thick. Volatile organic solvent extraction was improved by vapor phase extraction (headspace method). The method was validated in terms of its main characteristics. Parameters for the suitability of the chromatography system were developed. Batch samples of kemantane substance were analyzed.

Keywords

kemantane 5-hydroxyadamantan-2-one pharmaceutical substance residual organic solvents vapor phase extraction (headspace method) validation gas liquid chromatography GLC 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • A. V. Tolkacheva
    • 1
    • 2
  • L. N. Grushevskaya
    • 1
  • N. I. Avdyunina
    • 1
  • B. M. Pyatin
    • 1
  • V. I. Prokof’eva
    • 2
  • L. M. Gaevaya
    • 1
  • N. M. Zaitseva
    • 1
  1. 1.V. V. Zakusov Science Research Institute of PharmacologyMoscowRussia
  2. 2.I. M. Sechenov First Moscow Medical UniversityMoscowRussia

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