Advertisement

Pharmaceutical Chemistry Journal

, Volume 48, Issue 12, pp 815–818 | Cite as

Possible Production of Prothrombin Complex Concentrate by Anion-Exchange Column Chromatography

  • A. V. Iserkapov
  • A. L. Berkovskii
  • A. V. Slobodyan
  • T. L. Dereza
  • O. G. Kutyurova
  • N. S. Shastina
  • V. I. Shvets
DRUG SYNTHESIS METHODS AND MANUFACTURING TECHNOLOGY

Methods for producing prothrombin complex concentrate (PPSB) by anion-exchange chromatography in batch mode using various sorbents such as DEAE- and QAE-Sephadex A-50 were examined. It was established that the yield and specific activity of FII, FVII, FIX, and FX depended on the mass of resin used for chromatography. New column chromatography methods for producing PPSB with a high FVII content were proposed. The static capacity of several sorbents for coagulation system FVII and the dynamic capacity of TOYOPEARL GigaCap Q-650M and Capto Q were studied.

Keywords

prothrombin complex concentrate column chromatography 

References

  1. 1.
    V. M. Rusanov and I. Levin, Medicinal Blood Preparations [in Russian], ID Medpraktika, Moscow (2004), pp. 55 – 56.Google Scholar
  2. 2.
    M. M. Song, C. P. Warne, and M. A. Crowther, Thromb. Res., 129(4), 526 – 529 (2012).CrossRefPubMedGoogle Scholar
  3. 3.
    R. Demeyere, S. Gillardin, J. Arnout, et al., Vox Sang., 99(3), 251 – 260 (2010).CrossRefPubMedGoogle Scholar
  4. 4.
    J. Romisch and K. Pock, in: Production of Plasma Proteins for Therapeutic Use, J. Bertolini, N. Goss, and J. Curling (eds.), John Wiley & Sons Inc., Hoboken, New Jersey (2012), pp. 65 – 79.CrossRefGoogle Scholar
  5. 5.
    A. A. Kozlov, A. L. Berkovskii, N. D. Kachalova, et al., Diagnostic Kits and Reagents for Hemoglobinometry and Hemostasis Systems [in Russian], Set of Instructions, NPO RENAM, Moscow (2010), pp. 11 – 21.Google Scholar
  6. 6.
    A. A. Kozlov, L. V. Natrus, P. A. Chernovol, et al., Laboratory Hemostasis Diagnostic System [in Russian], Litterra, Moscow (2011), pp. 91 – 98.Google Scholar
  7. 7.
    N. J. Kruger, The Bradford method for protein quantitation, Methods of Enzymology. Basic protein and peptide protocols, J. M. Walker (ed.), Humana Press Inc., Totowa, New Jersey (1994), Vol. 32, pp. 9 – 15.CrossRefGoogle Scholar
  8. 8.
    European Pharmacopoeia, 4th Ed. (2002), pp. 1325 – 1326.Google Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • A. V. Iserkapov
    • 1
    • 2
  • A. L. Berkovskii
    • 1
  • A. V. Slobodyan
    • 1
    • 2
  • T. L. Dereza
    • 1
  • O. G. Kutyurova
    • 1
  • N. S. Shastina
    • 2
  • V. I. Shvets
    • 2
  1. 1.Scientific Hematological Center, Russian Ministry of HealthMoscowRussia
  2. 2.M. V. Lomonosov Moscow State University of Fine Chemical TechnologyMoscowRussia

Personalised recommendations