Pharmaceutical Chemistry Journal

, Volume 48, Issue 11, pp 727–732 | Cite as

Chemical Constituents and In Vitro Anticancer, Cytotoxic, Mutagenic and Antimutagenic Activities of Artemisia Diffusa

  • M. Taherkhani

Water-distilled essential oil from the leaves of Artemisia diffusa Krasch. ex Poljakov, collected from north east of Iran, was investigated for phytochemical constituents and anticancer, cytotoxic, mutagenic and antimutagenic activities. Camphor (28.30%), 1,8-cineole (21.03%) and β-thujone (14.20%) were the major components in this oil. The largest part of the leaf oil of A. diffusa was formed by oxygenated monoterpenes (75.58%). Cytotoxicity was measured using a modified MTT assay against Hela and lymphocyte cells. The IC50 shows that cytotoxicity of the oil with respect to human tumor cell line (IC50 = 16.34 μg/mL) is much higher than that for healthy human cells (IC50 = 4594.92 μg/mL). These results indicate low adverse side effects of the oil. The mutagenic and antimutagenic activities of the A. diffusa oil were evaluated by the Ames Salmonella/microsome assay, using the Salmonella typhimurium tester strains TA98 and TA100, with and without the presence of metabolic activation of rat liver (S9). The excellent anti-mutagenic effect was seen in 1.16 mg/plate against both strains of S. typhimurium TA100 and TA98, without the presence of S9 fraction.


Artemisia diffusa camphor cytotoxicity MTT assay Ames Salmonella/microsome test anti-mutagenic agent 



I wish to thank the Islamic Azad University’s (Takestan Branch) research deputy office for the sanction of research grant to conduct the current research (Grant No. TIAU: 50155). I would like to thank sincerely Dr. Iraj Rasooli in the medicinal plant research center of Shahed University for scientific assistance. I would also like to thank Dr. Mozaffarian for help in identifying plant material. I am grateful to Tofigh Taherkhani for helpful assistance in botanical collection.


  1. 1.
    K. H. Rechinger, Artemisia, in: Flora Iranica, Compositae, No. 158, K. H. Rechinger and I. C. Hedge (eds.), Akademische Druck und Verlagsanstalt, Gras, Austria (1986).Google Scholar
  2. 2.
    V. A. Mozaffarian, Dictionary of Iranian Plant Names, Farhang Moaser, Tehran, Iran (1996).Google Scholar
  3. 3.
    A. Rustaiyan and S. Masoudi, Phytochem. Lett. 4, 440 – 447 (2011).CrossRefGoogle Scholar
  4. 4.
    A. Firouzni, H. Vahedi, F. Sabbaghi, and M. Bigdeli, Chem. Nat. Comp., 44, 804 – 806 (2008).CrossRefGoogle Scholar
  5. 5.
    R. P. Adams, Identification of Essential Oil Components by Gas Chromatography / Quadrupole Mass Spectroscopy, Allured Publ. Corp., Carol Stream, IL (2001).Google Scholar
  6. 6.
    M. Hassanzadeh Khayyata and H. Karimi, Iran J. Pharm. Sci., 1(1), 33 – 37 (2004).Google Scholar
  7. 7.
    K. Khazraei-Alizadeh and A. Rustaiyan, J. Essent. Oil Res., 13(3), 185 – 186 (2001).CrossRefGoogle Scholar
  8. 8.
    A. Rustaiyan, H. Nahrevanian, and M. Kazemi, Pharmacogn. Mag., 5(17), 1 – 7 (2009).Google Scholar
  9. 9.
    S. M. Sharafi, I. Rasooli, P. Owlia, M. Taghizadeh, and S. Darvish Alipoor Astaneh, Pharmacogn. Mag., 6(23), 147 – 153 (2010).CrossRefPubMedCentralPubMedGoogle Scholar
  10. 10.
    M. Sylvestre, J. Legault, D. Dufour, and A. Pichette, Phytomedicine, 12(4), 299 – 304 (2005).CrossRefPubMedGoogle Scholar
  11. 11.
    K. Mortelmans and E. Zeiger, Mutat. Res., 455(1 – 2), 29 – 60 (2000).CrossRefPubMedGoogle Scholar
  12. 12.
    R. M. Samarth, M. Panwar, M. Kumar, and A. Kumar. Mutagenesis, 21(1), 61 – 66 (2006).CrossRefPubMedGoogle Scholar
  13. 13.
    J. R. Lazutka, J. Mierauskiene, G. Slapsyte, and V. Dedonyte, Food Chem. Toxicol., 39(5), 485 – 492 (2001).CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Chemistry, Takestan BranchIslamic Azad UniversityTakestanIran

Personalised recommendations