Water-soluble polysaccharides (WSPS) were isolated from Calendula officinalis flowers using aqueous extraction followed by precipitation with EtOH and dialysis through a semi-permeable membrane. The WSPS were standardized with respect to uronic acid, protein, and nucleic acid content. Total WSPS were separated into five main components (PSC-1, PSC-2, PSC-3, PSC-4, PSC-5) by ion-exchange chromatography over DEAE cellulose. The main components in WSPS from C. officinalis were acidic polysaccharides (rhamnogalactouronan I).
Calendula officinalis L. water-soluble polysaccharides calendula flowers
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