Pharmaceutical Chemistry Journal

, Volume 45, Issue 1, pp 47–50 | Cite as

Identification of flavonoids in plants by HPLC

  • D. V. Moiseev
  • G. N. Buzuk
  • V. L. Shelyuto

The HPLC capacity factors of 27 flavonoids were determined using the following chromatographic conditions: Zorbax SB 250 × 4.6 mm analytical columns containing C-18, C-8, and CN sorbents with particle size 5 μm, mobile phase consisting of 0.01 M potassium dihydrogen phosphate pH 3.0 and acetonitrile at a ratio of 80:20 (v/v), column temperature 30°C; absorption spectra were recorded over the range 190 – 400 nm. This chromatography system can be used for preliminary identification of flavonoids in plant materials.

Key words

Flavonoids in plants identification by HPLC 


  1. 1.
    S. Ya. Sokolov, Phytotherapy and Phytopharmacology: Handbook for Doctors [in Russian], Medical Information Agency, Moscow (2000).Google Scholar
  2. 2.
    V. L. Shelyuto, Author’s Abstract of Doctoral Thesis in Pharmaceutical Sciences, Moscow (1988).Google Scholar
  3. 3.
    L. Y. Galichet (Ed.), Clarke’s Analysis of Drugs and Poisons, Third edition (electronic version), Pharmaceutical Press, London (2005).Google Scholar
  4. 4.
    T. J. Mabry, K. R. Markham, and M. B. Thomas, The Systematic Identification of Flavonoids, Springer Verlag, Berlin, Heidelberg, New York (1970).Google Scholar
  5. 5.
    V. F. Selemeneva (Ed.), The Chromatographer’s Companion. Methods for Liquid Chromatography [in Russian], Vodolei, Voronezh (2004).Google Scholar
  6. 6.
    V. D. Shatts and O. V. Sakhartova, High Performance Liquid Chromatography [in Russian], Zinatne, Riga (1988).Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2011

Authors and Affiliations

  • D. V. Moiseev
    • 1
  • G. N. Buzuk
    • 1
  • V. L. Shelyuto
  1. 1.Vitebsk State Medical UniversityVitebskBelarus

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