Gas chromatographic analysis of short-chain fatty acids in the standardization of medicinal formulations based on bacterial substrates
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Recent years have seen increasing use of formulations containing sets of compensatory metabolites in the form of short-chain fatty acids (SCFA), i.e., acetic, propionic, butyric, isobutyric, valeric, and isovaleric acids. This has led to the need to adapt existing analytical methods to monitor the presence of SCFA in probiotic formulations. The main task of the present study was to develop an assay for SCFA in formulations based on bacterial substrates. This was addressed using gas chromatography with flame ionization detection.
Key wordsGC (gas chromatography) probiotics short-chain fatty acids
- 1.B. A. Shenderov, Medical Microbial Ecology and Functional Nutrition [in Russian], Grant’ Press, Moscow (1998), Vol. 1, pp. 28 – 48.Google Scholar
- 2.M. D. Ardatskaya, Dissertation for Doctorate in Medical Sciences [in Russian], Moscow (2003).Google Scholar
- 3.D. P. Collin, P. G. McCormick, and M. G. Schmidt, J. Clin. Chem., 20, 1235 – 1237 (1974).Google Scholar