Pharmaceutical Chemistry Journal

, Volume 44, Issue 1, pp 47–50 | Cite as

Analysis and antibacterial activity of hemolymph fractions from immunized Galleria mellonella larvae

  • P. P. Purygin
  • O. S. Sribnaya
  • A. K. Buryak

The antibacterial activity of hemolymph fractions isolated from Galleria mellonella larvae has been studied. Protein fractions possessing antibacterial activity were obtained using ion-exchange chromatography and gel electrophoresis techniques. MALDI-TOF mass-spectrometric analysis of these fractions showed the presence of a peptide with molecular mass 5627 Da that probably accounts for the antibacterial activity of the hemolymph. The obtained data confirm the expediency of using G. mellonella larvae hemolymph preparations (including homeopathic drugs) in medicine.

Key words

insect immunity Galleria mellonella MALDI of peptides gel electrophoresis 


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Copyright information

© Springer Science+Business Media, Inc. 2010

Authors and Affiliations

  • P. P. Purygin
    • 1
  • O. S. Sribnaya
    • 1
  • A. K. Buryak
    • 1
  1. 1.Samara State UniversitySamaraRussia

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