Drug synthesis methods and manufacturing technology
Alkaline protease from Bacillus subtilis and lysozyme from chicken egg white have been immobilized for the first time in poly(N-vinylpyrrolidone) modified by polysilicic acid sol. Elastic polymeric hydrogels that are insoluble under physiological conditions are obtained and provide potential wound-healing coatings with high retention of lytic and proteolytic activities (80 – 100%) and prolonged action (24 h) and complex preparations with proteolytic and 1.7-fold increased lytic activity. It is established that immobilized alkaline protease and lysozyme are stable under conditions modeling the wound pH level during storage for one year and upon sterilization by γ-irradiation.
Key wordsalkaline protease lysozyme immobilization poly(N-vinylpyrrolidone)
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