Pharmaceutical Chemistry Journal

, Volume 42, Issue 12, pp 721–723 | Cite as

Evaluation of the purity of vitamin a oil-based preparations by thin-layer chromatography

  • O. V. Checheta
  • E. F. Safonova
  • A. I. Slivkin
  • G. A. Ogol’
Structure of Chemical Compounds, Methods of Analysis and Process Control

An important stage in pharmaceutical analysis is the estimation of the degree of purity of parent substances and related preparations. There is currently a need to develop simple, economic, and accessible methods for rapid analysis. One such method is thin-layer chromatography (TLC). In the present work, the possibility of using TLC for monitoring the content of impurities in oil-based vitamin A preparations and for their separation and identification is considered.


Dosage Form Retinol Parent Substance Main Spot Retinol Acetate 
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  1. 1.
    State Pharmacopoeia, XIth Ed., No. 2, Meditsina, Moscow (1990), pp. 41–45.Google Scholar
  2. 2.
    PS 42 – 2798 – 99, “Coated glutamevit tablets.”Google Scholar
  3. 3.
    A. P. Arbatskii, G. N. Afon’shin, and V. M. Vostokov, Zh. Anal. Khim., 59(12), 1304–1307 (2004).Google Scholar
  4. 4.
    L. V. Denisova, V. N. Filimonov, L. N. Balyatinskaya, et al., Zh. Anal. Khim., 52(9), 967–969 (1997).Google Scholar
  5. 5.
    S. A. Klyuev, Zh. Anal. Khim., 51(9), 961–963 (1996).Google Scholar
  6. 6.
    E. I. Kozlov, I. A. Solunina, M. L. Lyubareva, et al., Khim.-farm. Zh., 37(10), 50–53 (2003).Google Scholar
  7. 7.
    A. I. Luttseva, L. G. Maslov, and V. I. Seredenko, Khim.-farm. Zh., 35(10), 41–45 (2001).Google Scholar
  8. 8.
    M. Sarsunova, C. Michalec, and C. Schwarz, Thin-Layer Chromatography in Pharmacy and Clinical Biochemistry [in Slovak], Obzor, Bratislava (1968).Google Scholar
  9. 9.
    J. G. Kirchner, Techniques of Chemistry, Vol. 14: Thin-Layer Chromatography, 2nd Ed., Wiley-Interscience, New York (1978).Google Scholar
  10. 10.
    O. V. Rybakova, E. F. Safonova, and A. I. Slivkin, in: Abstracts of Papers of the IIIrd All-Russian Conference Pharmacy Education 2007 [in Russian], Voronezh (2007), part 1, pp. 301–303.Google Scholar
  11. 11.
    F. Geiss, Fundamentals of Thin Layer Chromatography, A. Huthig, Heidelberg, New York (1987).Google Scholar
  12. 12.
    PS 42-7811-97, “Solution of retinol acetate in oil 33,000 ME in capsules.”Google Scholar
  13. 13.
    SP Xth Ed., Meditsina, Moscow (1968), pp. 588–589.Google Scholar
  14. 14.
    European Pharmacopoeia, 3rd Ed., (2001), pp. 1590–1596.Google Scholar
  15. 15.
    G. A. Melent’eva, Pharmaceutical Chemistry of Certain Natural Substances with Strong Biological Activity [in Russian], Izd. Med. Inst. im. I. M. Sechenova, Moscow (1964), pp. 48–56.Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2008

Authors and Affiliations

  • O. V. Checheta
    • 1
  • E. F. Safonova
    • 1
  • A. I. Slivkin
    • 1
  • G. A. Ogol’
    • 1
  1. 1.Voronezh State UniversityVoronezhRussia

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