Quantitative estimation of components of combined hormonal contraceptives by HPLC
Published methods for the quantitative estimation (colorimetry, UV spectrophotometry, and fluorimetry) of the active components of oral contraceptives do not meet specificity requirements and do not allow sufficiently accurate monitoring of the estrogen and gestagen contents in therapeutic formulations. The present report describes identification of the optimum chromatographic conditions and development of a unified HPLC method for the identification and quantitative estimation of the active substances — ethinylestradiol, levonorgestrel, and gestodene — used in oral contraceptives.
KeywordsOral Contraceptive Levonorgestrel Ethinylestradiol Medroxyprogesterone Acetate Cyproterone Acetate
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