Pharmaceutical Chemistry Journal

, Volume 42, Issue 3, pp 153–156 | Cite as

Quantitative HPLC determination of phenolic compounds in yarrow

  • R. Benetis
  • J. Radusiene
  • V. Jakstas
  • V. Janulis
  • G. Puodziuniene
  • A. Milasius


The qualitative and quantitative compositions of flavonoids (vicenin-2, luteolin-3′-di-O-glycoside, luteolin-7-O-glycoside, rutin, apigenin-7-O-glycoside, luteolin, apigenin) and chlorogenic acid in herb, leaves, and stems of yarrow (Achillea millefolium) samples collected in the Kaunas region of Lithuania have been studied by means of high-performance liquid chromatography (HPLC). It is established that these substances exhibit a characteristic distribution with predominant formation of apigenin-7-O-glycoside and luteolin-7-O-glycoside among the identified flavonoids in the herb of yarrow whereas the leaves contain predominantly chlorogenic acid, rutin, and apigenin-7-O-glycoside; the stems, predominantly chlorogenic acid, rutin, apigenin-7-O-glycoside, and luteolin-7-O-glycoside. The results can be used to choose selection donors of this medicinal plant material.


Flavonoid Rutin Chlorogenic Acid Luteolin Apigenin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    X. T. Si, M. L. Zhang, Q. W. Shi, and H. Kiyota, Chem. Biodivers, 3, No. 11, 1163–1180 (2006).PubMedCrossRefGoogle Scholar
  2. 2.
    J. Barnes, L. A. Anderson, and J. D. Phillipson, Herbal Medicines, A Guide for Healthcare Professionals, Pharmaceutical Press, London, Chicago (2002), pp. 489–491.Google Scholar
  3. 3.
    M. Blumenthal, A. Goldberg, and J. Brinckmann, Herbal Medicine, Expanded Commission E Monographs, Integrative Medicine Communications, Newton (2000), pp. 419–423.Google Scholar
  4. 4.
    M. D. Garcia, R. Puerta, S. Martinez, and M. T. Saenz, Phytother. Res., 11, No. 5, 376–379 (1997).CrossRefGoogle Scholar
  5. 5.
    I. Aljancic, V. Vajs, N. Menkovic, et al., J. Nat. Prod., 62, No. 6, 909–911 (1999).PubMedCrossRefGoogle Scholar
  6. 6.
    B. Benedek, N. Geisz, W. Jager, et al., Phytomedicine, 13, No. 9–10, 702–706 (2006).PubMedCrossRefGoogle Scholar
  7. 7.
    G. Innocenti, E. Vegeto, S. Dall(acqua, et al., Phytomedicine, 14, No. 2–3, 147–152 (2007).PubMedCrossRefGoogle Scholar
  8. 8.
    S. Trifunovic, V. Vajs, Z. Juranic, et al., Phytochemistry, 67, No. 9, 887–893 (2006).PubMedCrossRefGoogle Scholar
  9. 9.
    E. Nemeth, J. Essent. Oil Res., 17, 501–512 (2005).Google Scholar
  10. 10.
    R. F. Chandler, S. N. Hooper, and M. J. Harvey, Econ. Bot., 36, No. 2, 203–223 (1982).Google Scholar
  11. 11.
    B. Benedek, E. Rozema, N. Gjoncaj, and B. Kopp, Z. Phytother., 27, S1, V02 (2006).Google Scholar
  12. 12.
    B. Weisshaar and G. I. Jenkins, Curr. Opin. Plant Biol., 1, No. 3, 251–257 (1998).PubMedCrossRefGoogle Scholar
  13. 13.
    European Pharmacopoeia, Suppl. 5.7 to the 5th Ed. 04 / 2007, Strasbourg, Council of Europe, 67075 (2006), p. 5145.Google Scholar
  14. 14.
    State Pharmacopoeia [in Russian], XIth Ed., Vol. 2, Meditsina, Moscow (1989), pp. 325–327.Google Scholar
  15. 15.
    Practicum on Agrochemistry [in Russian], Izd. Mosk. Univ., Moscow (1989).Google Scholar
  16. 16.
    GOST 26107-84, “Methods for determining total nitrogen.”Google Scholar
  17. 17.
    GOST 26207-84, “Determination of mobile forms of phosphorus and potassium by the CINAO modified Kirsanov method.”Google Scholar
  18. 18.
    GOST 26213-84, “Determination of humus by the CINAO modified Turin method.”Google Scholar
  19. 19.
    J. Radusiene and V. Janulis, Medicina (Meditsina, Kaunas), 40, No. 8, 705–709 (2004).PubMedGoogle Scholar
  20. 20.
    S. C. Grace and B. A. Logan, Philos. Trans. R. Soc. London, Ser. B:, 355, No. 1402, 1499–1510 (2000).PubMedCrossRefGoogle Scholar
  21. 21.
    R. Feng, Y. Lu, L. L. Bowman, et al., J. Biol. Chem., 280, No. 30, 27888–27895 (2005).PubMedCrossRefGoogle Scholar
  22. 22.
    O. Firuzi, L. Giansanati, R. Vento, et al., J. Pharm. Pharmacol., 55, No. 7, 1021–1027 (2003).PubMedCrossRefGoogle Scholar
  23. 23.
    M. D. dos Santos, M. C. Almeda, N. P. Lopes, and G. E. de Souza, Biol. Pharm. Bull., 29, No. 11, 2236–2240 (2006).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2008

Authors and Affiliations

  • R. Benetis
    • 1
  • J. Radusiene
    • 1
  • V. Jakstas
    • 1
  • V. Janulis
    • 1
  • G. Puodziuniene
    • 1
  • A. Milasius
    • 1
  1. 1.Kaunas University of MedicineKaunasLithuania

Personalised recommendations