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Chemical composition of dry extracts of Gymnema sylvestre leaves

  • V. A. Khramov
  • A. A. Spasov
  • M. P. Samokhina
Article

Abstract

Three certified preparations of dried extracts of Gymnema sylvestre with gravimetric concentrations of “25%,” “50%,” and “75%” were studied. After extraction with a water-alcohol mixture, the supernatants of all preparations were analyzed for the presence of phenolic acids and amino acids by ascending thin-layer chromatography. The samples were found to contain significant quantities of a diversity of organic compounds, including plant phenols. The presence of the latter was supported by the reaction with iron (III) chloride and the Pauli diazo reaction. The ninhydrin reaction showed that the samples contained some quantities of amino acids. These were greatest in the 50% preparation (0.15 mol/kg). Gymnema sylvestre was found to contain an interesting amino acid composition, in that there was no proline. According to the certification, the molar concentration of gymnemic acids in properties was 0.12 mol/kg, which was consistent with the concentrations of phenolic acids and amino acids identified in the present studies. Thus, purification of dry extracts of Gymnema sylvestre to obtain pure preparations of gymnemic acids requires procedures to reduce the contents of satellite compounds.

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Copyright information

© Springer Science+Business Media, Inc. 2008

Authors and Affiliations

  • V. A. Khramov
    • 1
  • A. A. Spasov
    • 2
  • M. P. Samokhina
    • 2
  1. 1.Volgograd State Technical UniversityVolgogradRussia
  2. 2.Volgograd State Medical UniversityVolgogradRussia

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