Pharmaceutical Chemistry Journal

, Volume 41, Issue 6, pp 289–293 | Cite as

Immunoglobulin: DNA cofactor interaction

  • M. K. Malinka
  • V. M. Petriev
  • V. K. Podgorodnichenko
Molecular Biological Problems of Drug Design and Mechanisms of Drug Action


The reasons for the appearance of DNA-binding activity of immunoglobulins (Ig) after chromatography on anion-exchange Sephadexes were studied. Elution of Ig from these Sephadexes with 0.5 M NaCl was found, using an orcinol method, to be associated with degradation of the matrix, with the appearance of water-soluble fragments (WSF) of Sephadex in the eluate. Degradation of the Sephadex matrix also occurred on washing of unloaded Sephadex. Monoclonal antibodies which did not initially interact with DNA acquired the ability to react with DNA after incubation with Sephadex WSF, as demonstrated using immunoenzyme analysis. It is suggested that the WSF forming on passage of Ig through anion exchange Sephadex columns interact with Ig to generate cations which can react with negatively charged antigens and, particularly DNA.


DEAE Anion Exchange Orcinol Cationic Property Start Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    V. K. Podgorodnichenko, A. M. Poverennyi, and D. B. Dzhumaev, Vopr. Med. Khim., 5, 63–67 (1982).Google Scholar
  2. 2.
    V. A. Saenko, G. M. Rott, and A. M. Poverennyi, Byull. Éksp. Biol. Med., 2, 217–220 (1989).Google Scholar
  3. 3.
    A. M. Poverennyi, G. M. Rott, and N. I. Sulaeva, Mol. Biol., 23(1), 153–164 (1989).Google Scholar
  4. 4.
    I. V. Lekakh, N. V. Bovin, and G. P. Bezyaeva, Biokhimiya, 66(2), 205–210 (2001).Google Scholar
  5. 5.
    V. A. Saenko and A. M. Poverennyi, Byull. Éksp. Biol. Med., 6, 624–625 (1993).Google Scholar
  6. 6.
    G. M. Rott, V. K. Podgorodnichenko, and V. S. Deeva, Vopr. Med. Farm. Khim., 2, 45–50 (2001).Google Scholar
  7. 7.
    G. M. Rott, V. K. Podgorodnichenko, and I. V. Rozhinskaya, Mol. Biol., 28, 383–391 (1994).Google Scholar
  8. 8.
    V. S. Deeva, Author’s Abstract of Thesis for Doctorate in Biological Sciences, Obninsk (1999).Google Scholar
  9. 9.
    D. N. Abakushin, Author’s Abstract of Thesis for Doctorate in Biological Sciences, Obninsk (1999).Google Scholar
  10. 10.
    Sephadex Ion Exchange. A Guide to Ion Exchange Chromatography, Pharmacia, Uppsala (1970).Google Scholar
  11. 11.
    R. L. Whistler and M. J. Wolf, Methods of Carbohydrate Chemistry [Russian translation], Moscow (1967).Google Scholar
  12. 12.
    I. V. Neverov and N. I. Titorenko, Lab. Delo, 6, 323–325 (1979).PubMedGoogle Scholar
  13. 13.
    D. Detterman, Gel Chromatography [Russian translation], Mir Press, Moscow (1970).Google Scholar
  14. 14.
    P. Galli, C. Confurius, and C. Maassen, Lancet, 335, 1544–1547 (1990).PubMedCrossRefGoogle Scholar
  15. 15.
    M. K. Malinka, V. M. Petriev, and V. K. Podgorodnichenko, Immunologiya, 1, 16–19 (2007).Google Scholar
  16. 16.
    V. M. Tishchenko, Biokhimiya, 65(10), 1450–1454 (2000).Google Scholar
  17. 17.
    Ya. I. Mel’nikova, S. G. Odintsov, and Z. I. Kravchuk, Biokhimiya, 65(11), 1488–1499 (2000).Google Scholar
  18. 18.
    M. A. Basharov, Biokhimiya, 65, 1400–1408 (2000).Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • M. K. Malinka
    • 1
  • V. M. Petriev
    • 1
  • V. K. Podgorodnichenko
    • 1
  1. 1.State Medical Radiological Scientific CenterRussian Academy of Medical SciencesObninsk, Kaluzhskaya Region

Personalised recommendations