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Prebiotic Vesicle Formation and the Necessity of Salts

  • Sarah E. Maurer
  • Gunarso Nguyen
Prebiotic Chemistry

Abstract

Self-assembly is considered one of the driving forces behind abiogenesis and would have been affected by the environmental conditions of early Earth. The formation of membranes is a key step in this process, and unlike large dialkyl membranes of modern cells the first membranes were likely formed from small single-chain amphiphiles, which are environment-sensitive. Fatty acids and their derivatives have been previously characterized in this role without concern for the concentrations of ionic solutes in the suspension. We determined the critical vesicle concentration (CVC) for three single-chain amphiphiles with increasing concentrations of NaCl. All amphiphile species had decreasing CVCs correlated to increasing NaCl concentrations. Decanoic acid and oleic acid were impacted more strongly than monoacylglycerol, likely because of electric shielding of the negatively charged headgroups in the presence of salt. There was no impact on the salt species as 100 mM NaBr, NaCl, and KCl all exhibited the same effect on CVC. This research shows the importance of salt in both the formation of life and in experimental design for aggregation experiments.

Keywords

Liposome Protocell Self-assembly Surfactant Critical vesicle concentration 

Notes

Acknowledgments

I would like to thank Pierre-Alain Monnard, who inspired much of this work through both his own research and his mentorship, and Tom Burkholder for his input and discussions. I would also like to thank the 2014-2015 Conecticut State Univeristy-American Association of University Professors (CSU-AAUP) Research grant for funding this work (Grant ID: ARMAUH).

References

  1. Apel CL, Deamer DW, Mautner MN (2002) Self-assembled vesicles of monocarboxylic acids and alcohols: conditions for stability and for the encapsulation of biopolymers. Biochim Biophys Acta Biomembr 1559(1):1–9CrossRefGoogle Scholar
  2. Buboltz JT, Feigenson GW (2005) Phospholipid solubility determined by equilibrium distribution between surface and bulk phases. Langmuir 21(14):6296–6301. doi: 10.1021/la047086k CrossRefPubMedGoogle Scholar
  3. Cape JL, Monnard P-A, Boncella JM (2011) Prebiotically relevant mixed fatty acid vesicles support anionic solute encapsulation and photochemically catalyzed trans-membrane charge transport. Chem Sci 2(4):661–671CrossRefGoogle Scholar
  4. Dixit NS, Mackay RA (1983) Absorption and emission characteristics of merocyanine-540 in microemulsions. J Am Chem Soc 105:2928–2929CrossRefGoogle Scholar
  5. Gebicki JM, Hicks M (1973) Ufasomes Are Stable Particles Surrounded by Unsaturated Fatty-Acid Membranes Nature 243:232–234Google Scholar
  6. Maurer SE, Deamer DW, Boncella JM, Monnard PA (2009) Chemical evolution of amphiphiles: glycerol monoacyl derivatives stabilize plausible prebiotic membranes. Astrobiology 9(10):979–987. doi: 10.1089/ast.2009.0384  CrossRefPubMedGoogle Scholar
  7. Maurer SE, Monnard PA (2011) Primitive Membrane Formation, Characteristics and Roles in the Emergent Properties of a Protocell Entropy-Switz 13:466–484. doi: 10.3390/e13020466
  8. Maurer SE, DeClue MS, Albertsen AN, Dorr M, Kuiper DS, Ziock H, Rasmussen S, Boncella JM, Monnard PA (2011) Interactions between catalysts and amphiphilic structures and their implications for a protocell model. ChemPhysChem 12(4):828–835. doi: 10.1002/cphc.201000843 CrossRefPubMedGoogle Scholar
  9. Monnard PA, Apel CL, Kanavarioti A, Deamer DW (2002) Influence of ionic inorganic solutes on self-assembly and polymerization processes related to early forms of life: Implications for a prebiotic aqueous medium Astrobiology 2:139–152Google Scholar
  10. Monnard PA, Deamer DW (2003) Preparation of vesicles from nonphospholipid amphiphiles. Liposomes Pt B 372:133–151CrossRefGoogle Scholar
  11. Morigaki K, Walde P, Misran M, Robinson BH (2003) Thermodynamic and kinetic stability. Properties of micelles and vesicles formed by the decanoic acid/decanoate system. Colloids Surf A 213(1):37–44CrossRefGoogle Scholar
  12. Namani T, Walde P (2005) From decanoate micelles to decanoic acid/dodecylbenzenesulfonate vesicles. Langmuir 21(14):6210–6219. doi: 10.1021/La047028z CrossRefPubMedGoogle Scholar
  13. Rasmussen S, Chen LH, Deamer D, Krakauer DC, Packard NH, Stadler PF, Bedau MA (2004) Transitions from nonliving to living matter. Science 303:963–965CrossRefPubMedGoogle Scholar
  14. Ruso JM, Attwood D, Taboada P, Mosquera V, Sarmiento F (2000) Light scattering and NMR studies on the self-aggregation of sodium n-hexyl sulfate in aqueous electrolyte solution. Langmuir 16(4):1620–1625. doi: 10.1021/la990721f CrossRefGoogle Scholar
  15. Shih Y-h, Zhuang C-m, Peng Y-H, Lin C-h, Tseng Y-m (2012) The effect of inorganic ions on the aggregation kinetics of lab-made TiO2 nanoparticles in water. Sci Total Environ 435–436:446–452. doi: 10.1016/j.scitotenv.2012.06.076 CrossRefGoogle Scholar
  16. Tanford C (1973) The hydrophobic effect: formation of micelles and biological membranes. Wiley, New YorkGoogle Scholar
  17. Xie W-H, Shiu W-Y, Mackay D (1997) A review of the effect of salts on the solubility of organic compounds in seawater. Mar Environ Res 44:429–444CrossRefGoogle Scholar
  18. Zhai L, Tan X, Li T, Chen Y, Huang X (2006) Influence of salt and polymer on the critical vesicle concentration in aqueous mixture of zwitterionic/anionic surfactants. Colloids Surf A 276(1–3):28–33. doi: 10.1016/j.colsurfa.2005.09.043 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Department of Chemistry and BiochemistryCentral Connecticut State UniversityNew BritainUSA

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