The numerical prediction of the characteristics of directional multimodal couplers for two-photon endoscopy
- 369 Downloads
The first demonstration of 3D imaging using two-photon excitation through a multimode optical fibre took place in 2015 but the presented experimental setup contained a lot of bulk optics which is inconvenient in use. This paper presents a numerical analysis of power distribution transformations in multimode optical fibre couples for the purpose of developing couplers in nonlinear optical systems. The numerical results are proven by experimental results.
KeywordsMultimodal couplers Two-photon endoscopy Power distribution transformations
There are numerous well known nonlinear diagnostic systems used to examine the tissues of the sick. Most frequently single-mode fibres are used in nonlinear systems to deliver an ultrafast signal to the tested sample without any deformations. However, there is an increasing number of works presenting attempts to use multimode fibres in non-linear imaging systems. Multimode fibres usually have a thickness of a few micrometres and thanks to the fact that many modes are propagated in them, they can replace thick laser beams (several mm) composed of many single-mode fibres. On the other hand the big amount of propagating modes generates random speckled patterns at the distal tip of the multimode fibre. Such a speckle field is formed as a result of the interference among all of the propagating modes. To control the propagation of these modes several methods such as wavefront shaping techniques (Čižmár and Dholakia 2012; Choi et al. 2012; Morales et al. 2016; Sivankutty et al. 2016) or a digital phase conjugation (Morales-Delgado et al. 2015a, b, 2016) are used. For example a nonlinear setup using a multimode fibre was first demonstrated in 2015 (Morales-Delgado et al. 2015a, b). In this work a 20-cm section of a multimode fibre was used for pulse signal transfer. Signal fuzziness was compensated by a special system in which the selective phase conjugation method was used (Morales-Delgado et al. 2015a, b), thanks to this modes with similar group velocities are excited. Selectively excited modes are forced to follow the same route inside a fibre and next they constructively interfere on the fibre output to form a spot of minimum time fuzziness. Using digital phase conjugation it is possible to obtain 1–3 µm lateral resolution (Papadopoulos et al. 2013). Another technic which is used to control the propagation of the modes in the multimode fibre relays on using an orthonormal set of illumination pattern (Čižmár and Dholakia 2012). It was also shown that it is possible to predict the banding angle based on the analysis of the transmission matrix of the fibre and use it for image correction (Plöschner et al. 2015). Recently a very interesting approach was shown where a special light modulator shapes the input excitation wavefront to focus light on the distal tip of the multimode fibre (Caravaca-Aguirre and Piestun 2017). In aforementioned paper the authors demonstrated that proper selection of the multimode fibre is critical for a robust calibration and for high signal-to background ratio performance. The authors obtained the best results for graded- index fibres. In our approach we would like to simplify the construction of an endoscopy setup using fibre couplers made with multimode graded-index fibres. To achieve this goal it is necessary to develop appropriate couplers and a method which would enable forecasting power distribution on the output of the above mentioned couplers. The work presents the numerical analysis of power distribution transformation in multimode couplers and proposes a polished multimode coupler for research on single- and multi-photon fluorescence.
2 The calculations
The numerical examination of power distribution in the X coupler is based on the method proposed by Frieden (1980). As it has not been widely applied to date (to our knowledge), the Authors will present a short outline.
The intensity distribution within the multimode fibre may be referred to as the joint distribution of a set of random variables. Whatever the light source is, one has to take into account its spatial and angular emission probability density. In the following considerations the energy coefficients assigned to the rays at particular locations and directions will be proportional to the distribution densities of respective random variables. We assume the Gaussian distribution of the light source in the input (port 1).
By the law of large numbers the above densities of random variables closely approximate the corresponding densities of ray strikes.
Thus, one obtains the numerical values of respective differentials. For sufficiently small values of dxi, …, dγi the partial derivatives of Eq. (7) may now be approximated. Each such pair of numerically traced rays yields one point on the output spot intensity curve which is the merit of the whole procedure.
One strong requirement appears when choosing the ray racing method: it must be accurate to a very high degree. This is an indispensable condition—the whole procedure must be sensitive to minute variations between the ray and its differential counterpart. From among a number of practical procedures for numerical ray tracing, the Authors chose two methods: one proposed by Sharma et al. (1982) and the one proposed by Heimrath (1985). Both methods yield similar results, however, Sharma’s method based on the Runge–Kutta integration of differential equations offered faster convergence and the results presented below were obtained by this procedure.
3 The measurements
The Authors examined the X-couplers manufactured at the Wroclaw University of Science and Technology, Poland. The couplers were made using the side polishing method to facilitate the evaluation of the distribution of power propagation. The main components of the couplers were the multimode GI optical fibres (50/125 core/clad diameters). During experiments and numerical analysis we considered 0.5 m long fibres. The fibre branches were fitted into the supporting glass cubes with a rift of radius curvature R and then polished to depth h (see Fig. 1). Both similar components of the coupler were then mounted together with an immersion liquid of refraction index n i , and thickness d i . The distance between the centres of cores depends on both polishing depths and immersion film thickness and is referred to as d. The relative longitudinal shift of the centres of the polished zones in both branches is referred to as Δz. This process allows for relatively easy and accurate control of the dividing factor.
The output beam was detected and measured by means of photodiodes in a homodyne setup.
The Authors calculated the coupling coefficients or normalized power P for a number of possible configurations. In all cases the radius R of fibre curvature was fixed at 125 mm. One of the most interesting results is the prediction of the normalized power and its dependence on such technical parameters as immersion liquid index n i and polishing depth h.
The presented evidence leads to the conclusion that the proposed method of numerical simulation enables a relatively easy and inexpensive (once the computer program is executable) way of predicting the optical characteristics of multimode, gradient-index of optical components. The analysis was limited to X couplers only, but the method may be tested against other devices as well. These results are promising toward the implantation of such methods in predicting light distribution on the ports of the multimode GI coupler. In the next manuscript we would like to consider also phase of the lunched signals in our numerical model and predict the speckle pattern on the port 4 of the investigated coupler. Using a properly designed coupler can significantly simplify a two photon endoscopy setup.
Funding was provided by Politechnika Wrocławska (Grant No. 0401/0105/2016). The calculations were carried out in the Wroclaw Centre for Networking and Supercomputing (http://www.wcss.wroc.pl), Grant No. 184.
- Heimrath, A.: Numeryczna analiza biegu promieni w światłowodach gradientowych grubych. Politechnika Wrocławska, Wrocław (1985)Google Scholar
- Morales Delgado EE, Psaltis D, Moser C (2016) Two-photon excitation endoscopy through a multimode optical fibre. In: Proceedings of SPIE 9717, Adaptive Optics and Wavefront Control for Biological Systems II, p. 97171E. 15 March 2016Google Scholar
- Morales E, Psaltis D, Moser C (2016) Focusing and scanning of femtosecond pulses through a multimode fibre: applications in two-photon imaging and polymerization. In: brak miejsca, Optical Society of America, p. AT5C.1Google Scholar
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.