Photosensitizer doped colloidal mesoporous silica nanoparticles for three-photon photodynamic therapy
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Multiphoton microscopy and nanoparticle-assisted photodynamic therapy are two important areas in biophotonics research. In this paper, we combined these two technologies together. Photosensitizer (HPPH) doped colloidal mesoporous silica nanoparticles were synthesized and systematically characterized. Based on a confocal microscope equipped with a 1560 nm femtosecond laser, we showed: (1) direct three-photon luminescence from HPPH doped nanoparticles, which were uptaken by tumor cells, and (2) cytotoxicity towards tumor cells by HPPH under three-photon excitation. Nanoparticle-assisted three-photon photodynamic therapy can be a promising technology for disease diagnosis and in vivo/clinical therapy.
KeywordsMultiphoton processes Photodynamic therapy Nanomaterials
This work was supported by National Basic Research Program of China (973Program; 2011CB503700 and 2013CB834704), the National Natural Science Foundation of China (61275190), the Program of Zhejiang Leading Team of Science and Technology Innovation (2010R50007), the Fundamental Research Funds for the Central Universities, and the Open Fund of the State Key Laboratory of Luminescent Materials and Devices (South China University of Technology).
- Choi, J., Burns, A.A., Williams, R.M., Zhou, Z., Flesken-Nikitin, A., Zipfel, W.R., Wiesner, U., Nikitin, A.Y.: Core-shell silica nanoparticles as fluorescent labels for nanomedicine. J. Biomed. Opt. 12(6), 064007 (2007)Google Scholar
- Henderson, B.W., Bellnier, D.A., Greco, W.R., Sharma, A., Pandey, R.K., Vaughan, L.A., Dougherty, T.J.: An in vivo quantitative structure-activity relationship for a congeneric series of pyropheophorbide derivatives as photosensitizers for photodynamic therapy. Cancer. Res. 57(18), 4000–4007 (1997)Google Scholar
- Kim, S., Ohulchanskyy, T.Y., Pudavar, H.E., Pandey, R.K., Prasad, P.N.: Organically modified silica nanoparticles co-encapsulating photosensitizing drug and aggregation-enhanced two-photon absorbing fluorescent dye aggregates for two-photon photodynamic therapy. J. Am. Chem. Soc. 129(9), 2669–2675 (2007a)CrossRefGoogle Scholar
- Law, W.C., Yong, K.T., Roy, I., Xu, G., Ding, H., Bergey, E.J., Prasad, P.N.: Optically and magnetically doped organically modified silica nanoparticles as efficient magnetically guided biomarkers for two-photon imaging of live cancer cells. J. Phys. Chem. C 112(21), 7972–7977 (2008)CrossRefGoogle Scholar
- Prasad, P.N.: Introduction to Biophotonics. Wiley, New York (2004)Google Scholar
- Roy, I., Ohulchanskyy, T.Y., Pudavar, H.E., Bergey, E.J., Oseroff, A.R., Morgan, J., Prasad, P.N.: Ceramic-based nanoparticles entrapping water-insoluble photosensitizing anticancer drugs: a novel drug-carrier system for photodynamic therapy. J. Am. Chem. Soc. 125(26), 7860–7865 (2003)CrossRefGoogle Scholar