Tomographic Imaging of Collagen Fiber Orientation in Human Tissue Using Depth-Resolved Polarimetry of Second-Harmonic-Generation Light
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We propose a nonlinear optical probe method to image the distribution of collagen fiber orientation in human tissue by measuring the polarization of collagen-induced second-harmonic-generation (SHG) light (SHG polarimetry). Depth-resolved SHG polarimetry, with a depth resolution of 14 μm, was used to evaluate the cross-sectional profile of collagen fiber orientation in Achilles tendon and dentin, revealing a characteristic distribution of collagen orientation along the depth direction. We evaluated the two-dimensional (2D) lateral distribution of collagen fiber orientation in back reticular dermis and anklebone by polarization-resolved SHG imaging, and confirmed an appreciable difference in the distribution profiles of the two samples. We further extended the method to a depth-resolved measurement of the three-dimensional (3D) distribution of collagen orientation in anklebone. The proposed system promises to be a powerful tool for in vivo measurement of collagen fiber orientation in human tissue.
Keywordscollagen femtosecond laser orientation polarimetry second-harmonic-generation tomography
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- Centonze, V.E., White, J.G. 1998Biophys J.752015Google Scholar
- König, K., Riemann, I. 2003J. Biomed. Opt.8432Google Scholar
- Langton C.M., Njeh C.F. In: The Physical Measurement of Bone, Institute of Physics Publishing, London, 11, 2004.Google Scholar
- Stoller, P., Reiser, K.M., Celliers, P.M., Rubenchik, A.M. 2002Biophys. J.823330Google Scholar
- Yasui T., Shimabayashi K., Tohno Y., Araki T. In: The 5th Pacific Rim Conference on Lasers and Electro-Optics CLEO, Proc. The 5th Pacific Rim Conference on Lasers and Electro-Optics, TaiPei, 15–19 December 2003, 361, 2003.Google Scholar