Involvement of Supraoptic Astrocytes in Basilar Artery Occlusion-Evoked Differential Activation of Vasopressin Neurons and Vasopressin Secretion in Rats

Abstract

Vasopressin (VP) is a key factor in the development of brain injury in ischemic stroke. However, the regulation of VP secretion in basilar artery occlusion (BAO) remains unclear. To clarify the regulation of VP secretion in BAO and the underlying mechanisms, we performed this study in a rat model of BAO with (BC) or without common carotid artery occlusion (CCAO). The results showed that BAO and BC time-dependently increased neurological scores and that BC also increased water contents in the medulla at 2 h and in the pontine at 8 h. Moreover, plasma VP level increased significantly at BAO-8 h, CCAO and BC-2 h but not at BC-8 h; however, VP expressions increased in the supraoptic nucleus (SON) at BC-8 h. The neurological scores were highly correlated with pontine water contents and plasma VP levels. The number of phosphorylated extracellular signal-regulated protein kinase1/2-positive VP neurons increased significantly in the SON at BC-8 h. Similarly, the number of c-Fos-positive VP neurons increased significantly in the SON at BAO-8 h and BC-8 h. In addition, the length of glial fibrillary acidic protein (GFAP) filaments increased significantly in BC compared to BAO only. Aquaporin 4 (AQP4) puncta around VP neurons increased significantly at BC-8 h relative to BC-2 h, which had negative correlation with plasma VP levels. These findings indicate that BAO facilitates VP secretion and increases VP neuronal activity in the SON. The peripheral VP release is possibly under a negative feedback regulation of central VP neuronal activity through increasing GFAP and AQP4 expression in astrocytic processes.

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Abbreviations

AQP4:

Aquaporin 4

BAO:

Basilar artery occlusion

BC:

BAO plus CCAO

CCAO:

Common carotid artery occlusion

ERK1/2:

Extracellular signal-regulated protein kinase1/2

GFAP:

Glial fibrillary acidic protein

pERK1/2:

Phosphorylated ERK1/2

PVN:

Paraventricular nucleus

SON:

Supraoptic nucleus

VP:

Vasopressin

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Funding

This work was supported by the higher education talents funds of Heilongjiang province (grant No. 002000154, YFW) and the fund of “Double-First-Class” Construction of Harbin Medical University (key laboratory of preservation of human genetic resources and disease control in China).

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Contributions

DYL collected and analyzed data and wrote the first draft, DC participated the preliminary study, all participated in the discussion and revision of this work, and YFW designed the study and edited the draft.

Corresponding authors

Correspondence to Dongyang Li or Yu-Feng Wang.

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All authors claim that there are no conflicts of interest.

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Special issue: In Honor of Prof. Vladimir Parpura.

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Li, D., Cui, D., Jia, S. et al. Involvement of Supraoptic Astrocytes in Basilar Artery Occlusion-Evoked Differential Activation of Vasopressin Neurons and Vasopressin Secretion in Rats. Neurochem Res (2021). https://doi.org/10.1007/s11064-021-03246-9

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Keywords

  • Aquaporin 4
  • Glial fibrillary acidic protein
  • Ischemic stroke
  • Supraoptic nucleus
  • Paraventricular nucleus