Polyphenols from Toona sinensiss Seeds Alleviate Neuroinflammation Induced by 6-Hydroxydopamine Through Suppressing p38 MAPK Signaling Pathway in a Rat Model of Parkinson’s Disease

Abstract

Polyphenols from Toona sinensis seeds (PTSS) have demonstrated anti-inflammatory effects in various diseases, while the anti-neuroinflammatory effects still remain to be investigated. We aimed to investigate the effects of PTSS on Parkinson’s disease and underlying mechanisms using a rat model. We employed 6-hydroxydopamine (6-OHDA) to male Sprague Dawley (SD) rats and PC12 cells to construct the in vivo and vitro models of PD and dopaminergic (DA) neuron injury, respectively. Cell viability was detected by cell counting kit-8 (CCK-8) assay and protein levels of inflammatory mediators and some p38 MAPK pathway molecules were investigated by immunohistochemistry and Western blot analyses. The results showed that 6-OHDA significantly increased protein levels of inflammatory mediators, such as cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and tumor necrosis factor α (TNF-α), which could be reversed by PTSS through suppressing the p38 MAPK pathway. The anti-inflammatory effects of PTSS were significantly enhanced by the specific p38 inhibitor of SB203580 in vitro. The present work suggests that PTSS can exert anti-inflammatory effects on PD models, which may be attributed to the suppression of p38 MAPK signaling pathway.

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Acknowledgements

This research project was supported by the National Natural Science Foundation of China (Grant Number 81870943), the Shandong Province Natural Science Foundation of China (Grant NUMBER ZR2014HL043, and ZR2018LH006), the Shandong Province Science and Technology Development Program of China (Grant Number 2012GSF11827), the Shandong Provincial Education Department of China (Grant Number J11LF16), the Shandong Traditional Chinese Medicine Technology Development Project of China (Grant Number 2017-214), the Shandong Medical and Health Science and Technology Development Program of China (Grant Number 2015WS0063).

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Zhuang, W., Cai, M., Li, W. et al. Polyphenols from Toona sinensiss Seeds Alleviate Neuroinflammation Induced by 6-Hydroxydopamine Through Suppressing p38 MAPK Signaling Pathway in a Rat Model of Parkinson’s Disease. Neurochem Res (2020). https://doi.org/10.1007/s11064-020-03067-2

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Keywords

  • Parkinson’s disease
  • Neuroinflammation
  • Polyphenols from toona sinensis seeds
  • p38 MAPK