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Time-Course Changes and New Expressions of MIP-3α and Its Receptor, CCR6, in the Gerbil Hippocampal CA1 Area Following Transient Global Cerebral Ischemia

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Abstract

Macrophage inflammatory protein-3α (MIP-3α) and its sole receptor, CCR6, play pivotal roles in neuroinflammatory processes induced by brain ischemic insults. In this study, we investigated transient ischemia-induced changes in MIP-3α and CCR6 protein expressions in the hippocampal CA1 area following 5 min of transient global cerebral ischemia (tgCI) in gerbils. Both MIP-3α and CCR6 immunoreactivities were very strongly expressed in pyramidal neurons of the CA1 area from 6 h to 1 day after tgCI and were hardly shown 4 days after tgCI. In addition, strong MIP-3α immunoreactivity was newly expressed in astrocytes 6 h after tgCI. These results indicate that tgCI causes apparent changes in MIP-3α and CCR6 expressions in pyramidal neurons and astrocytes in the hippocampal CA1 area and suggest that tgCI-induced changes in MIP-3α and CCR6 expressions might be closely associated with neuroinflammatory processes in brain ischemic regions.

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Acknowledgements

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1D1A1B03029311), by the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MSIP (NRF-2015M3A9B6066968). and by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1D1A1B03030161).

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Author notes

  1. Joon Ha Park and YooHun Noh contributed equally to this article.

Authors and Affiliations

  1. Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, 24252, Republic of Korea

    Joon Ha Park & Ji Hyeon Ahn

  2. Famenity Company, Gwacheon, Geyonggi, 13837, Republic of Korea

    YooHun Noh & Sung-Su Kim

  3. Department of Emergency Medicine, and Institute of Medical Sciences, Kangwon National University Hospital, School of Medicine, Kangwon National University, Chuncheon, 24341, South Korea

    Taek Geun Ohk & Jun Hwi Cho

  4. Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, 24341, Republic of Korea

    Tae-Kyeong Lee, Hyunjung Kim, Minah Song, Jae-Chul Lee & Moo-Ho Won

  5. Department of Pharmacy, College of Pharmacy, Dankook University, Cheonan, Chungcheongnam-do, 31116, Republic of Korea

    Choong-Hyun Lee

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  1. Joon Ha Park
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Correspondence to Moo-Ho Won or Choong-Hyun Lee.

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Park, J.H., Noh, Y., Kim, SS. et al. Time-Course Changes and New Expressions of MIP-3α and Its Receptor, CCR6, in the Gerbil Hippocampal CA1 Area Following Transient Global Cerebral Ischemia. Neurochem Res 43, 2102–2110 (2018). https://doi.org/10.1007/s11064-018-2632-6

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  • DOI: https://doi.org/10.1007/s11064-018-2632-6

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