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Xueshuantong Injection (Lyophilized) Attenuates Cerebral Ischemia/Reperfusion Injury by the Activation of Nrf2–VEGF Pathway

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Abstract

Xueshuantong injection (Lyophilized, XST), extracted from the traditional Chinese medicinal herb Panax notoginseng, has neuroprotective effect on cerebral ischemia. Revascularization of ischemic tissue is good for the therapy of cerebrovascular disease. In this study, angiogenic potentiality and possible mechanism of XST for cerebral ischemia were explored. Rats were subjected to transient middle cerebral artery occlusion (MCAO), and then intraperitoneally administered with XST daily for 3 or 7 consecutive days. The neurological function deficits, and endogenous antioxidant capacity were evaluated. Post-stroke angiogenesis and vascularization were assessed by ELISA and immunohistochemical staining. Transcription levels of Nrf2, HO-1, NQO1 in brain tissues were analyzed by real-time RT-PCR. The results showed that XST could remarkably ameliorate neuronal functional deficit, promote angiogenesis and vascularization after MCAO. The mechanism of angiogenesis might be related to endogenous antioxidant capacity and Nrf2 pathway. In conclusion, administered XST for 7 days after stroke could significantly improve functional recovery and promote angiogenesis, that might be related to Nrf2 signaling pathway. These findings could provide scientific evidence for the use of XST in cerebral ischemic diseases and provide theoretical support for further studies.

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Acknowledgements

This research project was supported by the Key State Science and Technology Projects (2012ZX09101201-004), Tianjin Application Research Foundation (14JCYBJC28900), Guangxi Science and Technology Development Plan (14125008-2-5).

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Author notes

  1. Hong Guo, Dickson Adah and Peter Bai James have contributed to this article equally.

Authors and Affiliations

  1. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, #312 Anshanxi Road, Nankai District, Tianjin, 300193, China

    Hong Guo, Dickson Adah, Peter Bai James, Qingqing Liu, Guozheng Li, Peter Ahmadu, Lijuan Chai, Shaoxia Wang & Limin Hu

  2. Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China

    Hong Guo, Yang Liu & Limin Hu

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  1. Hong Guo
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  2. Dickson Adah
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  9. Yang Liu
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  10. Limin Hu
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Correspondence to Limin Hu.

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Guo, H., Adah, D., James, P.B. et al. Xueshuantong Injection (Lyophilized) Attenuates Cerebral Ischemia/Reperfusion Injury by the Activation of Nrf2–VEGF Pathway. Neurochem Res 43, 1096–1103 (2018). https://doi.org/10.1007/s11064-018-2523-x

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  • DOI: https://doi.org/10.1007/s11064-018-2523-x

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